Bcl-2、PCNA基因在鸡、鹌鹑及其杂交种睾丸组织中的表达研究

doi:10.16431/j.cnki.1671-7236.2017.07.021

Abstract

Abstract:

In order to investigate the molecular mechanism of stunted growth testis of chicken (♂) and quail (♀) hybrids. 84 testicular tissue samples of New Roman cocks, male Korean quails and chicken-quail hybrids at different developmental stages were collected,the mRNA expression of Bcl-2 and PCNA genes in testicular tissue of cocks, quails and chicken-quail hybrids at different growth stages were detected using Real-time PCR. The results showed that the Bcl-2 and PCNA genes mRNA expression patterns in testes of chickens and quails at different growth stages were similar.The Bcl-2 gene was fluctuated in a whole, and decreased to the lowest value, then recovered rapidly and remained at a high level. The PCNA gene had a very significant peak value.Compared with the chicken and quail,the expression of Bcl-2 and PCNA genes mRNA in hybrids had no obvious change, which indicated that the pattern of the Bcl-2 gene regulation apoptosis in testis was different from chicken and quail at the hybrid testicular development process, and there was no obvious cell appreciation process.It was suggested that the molecular influencing factors of testicular dysplasia of chicken and quail hybrids were related to the abnormal expression of Bcl-2 and PCNA genes mRNA in testes.The results provided an important reference for the further study of the mechanism of testicular dysplasia between chicken and quail interspecific hybrids.

Key words: chicken; quail; hybrid; testis; Bcl-2 gene; PCNA gene

CLC Number:

S813.2

ZHANG Mei, ZHOU Ling-ling, ZHANG Miao-miao, ZHONG Xin-xian, SHAO Jun, FAN Li-na, GENG Peng-rui, LI Yi-dan, LI Yan, LIAO He-rong. Study on Expression of Bcl-2 and PCNA Genes in Testicular Tissue of Chickens, Quails and Chicken-quail Hybrids[J]. , 2017, 44(7): 2050-2056.

References

[1] 耿鹏瑞,李岩,张金成,等. 鸡与鹌鹑属间杂交受精率的检测及其遗传性分析[J]. 西北农业学报,2013, 22(4):6-10.
[2] 孟祥东,严云勤. Bcl-2家族与线粒体对细胞凋亡的调控[J]. 细胞与分子免疫学杂志,2005,21(S1):77-79.
[3] Kelly P N, Strasser A. The role of Bcl-2 and its pro-survival relatives in tumourigenesis and cancer therapy[J]. Cell Death & Differentiation, 2011, 18(9):1414-1424.
[4] 宋楠萌,桑建利,徐恒. 增殖细胞核抗原(PCNA)的分子结构及其生物学功能研究进展[J]. 自然科学进展,2006, 16(10):1201-1209.
[5] Eguchi Y, Ewert D L, Tsujimoto Y. Isolation and characterization of the chicken bcl-2 gene:Expression in a variety of tissues including lymphoid and neuronal organs in adult and embryo[J]. Nucleic Acids Research, 1992, 20(16):4187-4192.
[6] Bonnefoy-Berard N, Aouacheria A, Verschelde C, et al. Control of proliferation by Bcl-2 family members[J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2004, 1644(2):159-168.
[7] Sanders E J, Parker E. Retroviral overexpression of bcl-2 in the embryonic chick lens influences denucleation in differentiating lens fiber cells[J]. Differentiation, 2003, 71(7):425-433.
[8] 周玲玲,李岩,梁杜,等. 鹌鹑早期胚胎bcl-2基因表达的差异及发育性变化[J]. 中国畜牧兽医,2010, 37(4):56-59.
[9] 乔爱君,李大全,马文霞,等. 鸡与鹌鹑属间杂交早期胚胎bcl-2、P53基因表达的差异及发育性变化[J]. 中国农业科学,2008,41(5):1497-1502.
[10] 王磊,张晗,张涛,等. 鸡胚心脏PCNA及AgNOR分布的研究[J]. 中国农业大学学报,2008,13(2):57-62.
[11] 徐丽华,刘春雷,常玉梅,等.双标准曲线相对定量PCR试验原理与方法[J].生物技术通报,2011,1:70-75.
[12] Straten P, Andersen M H. The anti-apoptotic members of the Bcl-2 family are attractive tumor-associated antigens[J]. Oncotarget,2010,1(4):239-245.
[13] Lian H D, Ren B, Gao X W. Effects of minocycline on expression of Bcl-2, bax in early retinal neuropathy of diabetes in rats[J]. International Journal of Ophthalmology, 2011, 4(2):162-164.
[14] Ketola I, Toppari J, Vaskivuo T, et al. Transcription factor GATA-6, cell proliferation, apoptosis, and apoptosis-related proteins Bcl-2 and Bax in human fetal testis[J].The Journal of Clinical Endocrinology & Metabolism, 2003, 88(4):1858-1865.
[15] 刘唯一,李岩,周红,等.鸡、鹌鹑及其杂交种早期胚胎Bcl-2基因甲基化的分析[J]. 中国畜牧兽医,2012,39(9):62-65.
[16] 廖和荣,李岩,郭晓令,等. ER、bcl-2和p53在鸡与鹌鹑属间杂交种早期胚胎中的mRNA表达[J].遗传,2008,30(7):907-912.
[17] 龙治峰,唐显庆,石金凤,等. 睾丸复位固定术对大鼠隐睾Bcl-2表达及生精细胞凋亡的影响[J]. 中南医学科学杂志,2013,41(3):229-232.
[18] 李洋洲,张大田. 人参皂苷Rg1对实验性精索静脉曲张大鼠睾丸生精细胞凋亡及Bcl-2、caspase-3表达的影响[J]. 中国生化药物杂志,2016,36(6):45-48.
[19] 刘国霖,张泽敏,唐圆圆,等. 大鼠精索静脉曲张对睾丸生精细胞Cx43表达的影响[J]. 北华大学学报(自然科学版),2016,17(5):624-627.
[20] Pan M, Kelman L M, Kelman Z. The archaeal PCNA proteins[J].Biochemical Society Transactions,2011, 9(1):20-24.
[21] Strasak A M, Kelleher C C, Brant L J, et al. Serum uric acid is an independent predictor for all major forms of cardiovascular death in 28,613 elderly women:A prospective 21-year follow-up study[J]. International Journal of Cardiology, 2008, 125(2):232-239.
[22] 郭海,才秀莲,王国秀.PCNA和Ki-67在染锰大鼠生精细胞中的表达及研究[J].河南职工医学院学报, 2013,25(5):544-548.
[23] 张飞虎,李健,靳冠通,等. 炔雌醚暴露对幼年小鼠睾丸发育及增殖细胞核抗原、半胱氨酸蛋白酶-3表达的影响[J]. 中国畜牧兽医,2014,41(3):199-202.
[24] 陈龙,杨国军,牛嗣云,等.人胎儿睾丸发生过程中TGF2β1与PCNA的表达[J].承德医学学院学报,2005,22(2):101-103.
[25] 牛嗣云,高福禄,庞小静,等.人胎儿睾丸发生过程中表皮生长因子与细胞增殖核抗原的表达[J].解剖学报,2003,34(6):645-648.
[26] 罗兰,吴艳瑞,张闻,等. PCNA基因在小鼠睾丸发育过程中的表达研究[J]. 云南大学学报(自然科学版),2016,5:820-826.

Study on Expression of Bcl-2 and PCNA Genes in Testicular Tissue of Chickens, Quails and Chicken-quail Hybrids

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	DU Yuanjun, DUAN Guochao, LIU Wei, LI Dapeng, HAN Haixia, LEI Qiuxia, ZHOU Yan, LIU Jie, WANG Jie, CAO Dingguo, LI Fuwei, CHEN Fu. Effect of Translucent on Egg Quality and Its Relationship with Intestinal Function of Laying Hen [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2305-2311.
[2]	WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.
[3]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[4]	ZHANG Huaqiang, WANG Pinsheng, LI Xiao, WEI Panpeng, YAN Lingmin, JIANG Bingbing, WANG Zhaogui, WANG Xuefang, ZENG Huanqing, FAN Yi, GAO Zhenglong, WANG Xuebing. Effects of Fermented Hybrid Broussonetia papyrifera on Growth Performance, Meat Quality,Digestive Enzyme Activity and Intestinal Flora Structure of Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1876-1887.
[5]	WU Jingxuan, SUN Zheng, MA Xuelian, ZHANG Liyang, LIAO Xiudong, LUO Xugang, LI Sufen, LYU Lin. A Survey on Magnesium Contents in Feed Resources for Livestock and Poultry in China [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1888-1898.
[6]	JIANG Shanyi, ZHOU Bijun, WEN Ming, WANG Kaigong. Prevention of Traditional Chinese Medicine with Probiotics on Eimeria tenella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2092-2102.
[7]	LIU Xin, CHEN Qun, QIU Yulang, LI Lin, HOU Guoxi, YAN Xiaogang, LI Zhonghe, CHEN Long, GAO Xingai, LIU Dongmei, ZHAI Di, YAN Qiuliang. Research Progress on the Production Mechanism and Control Technology of Chicken Manure Composting Nitrogen-containing and Sulfur-containing Waste Gases [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2166-2174.
[8]	DU Zhaohui, GE Zihao, YOU Junyi, LIU Xiaowei, LIANG Guodong, SUN Shiduo, HU Jianhong, LI Xiao. Effects of Clostridium butyricum and Sodium Butyricum on Reproductive System Injury Induced by High Fat Diet in Male Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1603-1616.
[9]	WU Zhi, ZHANG Cong, YUAN Huisha, DONG Hongyan, DAI Lixia, JIA Luqi, WU Shuang, ZHU Shanyuan. Isolation,Identification,Virulence and Drug Resistance of Salmonella from Yellow Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1695-1706.
[10]	HUANG Xiaoqian, YUAN Junzhao, LIU Jinhe, ZOU Hui, GU Jianhong, YUAN Yan, LIU Xuezhong, LIU Zongping, BIAN Jianchun. Protective Effect of Honokiol on Cadmium-induced Renal Injury in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1007-1015.
[11]	LIU Xin, PAN Jimai, HU Andong, ZENG Chengrong, YANG Ying, CHENG Zhentao, CHEN Jiangfeng, JIANG Haibo, WEN Ming. Effect of Astragalus Polysaccharides on Inactivated Aeromonas hydrophila Vaccine for Hybrid Sturgeon [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1177-1184.
[12]	DAN Yixin, XIANG Hua, ZHANG Huanrong, YANG Lu, REN Yupeng, XU Songwei, HE Honghong, ZHU Jiangjiang. Screening of Host Proteins Interacting with ORFV129 Protein and Cloning and Analysis of C1QBP Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 1-14.
[13]	LI Xinran, ZHANG Qingyan, ZHA Weiwei, ZHU Miao. Bioinformatics Analysis and Expression of Wnt Gene Family in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 15-25.
[14]	AN Xiaopeng, LIU Nan, HU Zhengjiamei, HU Jincao, XU Xiaolong, ZHANG Lei, SONG Yuxuan. Comparative Analysis of Lactation Performance of East Friesen Sheep, Hu Sheep and East Friesen-Hu Hybrid Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 238-245.
[15]	XIE Jingjing, REN Mengting, FAN Jiahui, JIAO Yaru, HAN Zongxi, MA Deying. Study on Immune Response of Infectious Bronchitis Virus to Chickens and Antiviral Effect of Recombinant Avian Beta-defensins 11 and 12 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 246-259.