China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (3): 84-86.

• 生物技术 • Previous Articles     Next Articles

Detection of Cattle-derived and Sheep-derived Component in Fresh Meat by T-RFLP Method

TIAN Jin-hui1，LI Bao-ming2，WEI Ting-yuan2，ZHOU Zhan-qin1，WU hui-juan2   

  1. (1.College of Animal S&T，Northwest A&F University，Yangling 712100，China；2.Beijiing Centre for Physical & Chemical Analysis，Beijing 100094，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-03-20 Published:2011-03-20
  • Contact: WU hui-juan

Abstract: To establish the qualitative analysis ways of fast testing the components of fresh sand mutton, taking strong measures against adulterations in the fresh meet. Using the T-RFLP method, the forward PCR primer was labeled with 6-carboxyfluorescein amino hexy(6-FAM) fluorescent dye and reverse PCR primer was labeled with ROX fluorescent dye after digestion by restriction enzyme HinfⅠand BlnⅠ. The restricition fragment were analysed by capillary electrophoresis using an antomated DNA sequencer. We can realize species identification through their different peak. Qualitative species identifying in the fresh beef and mutton were successfully identified by this rapid molecular technique. The PCR products of beef DNA which were digested with Hinf Ⅰ demonstrated 46 and 373 bp, meanwhile 203 and 515 bp for goat DNA which were digested with BlnⅠ.

Key words: COⅠ; PCR; terminal restriction fragment length polymorphism; cattle; goat

CLC Number: