钩棘单睾吸虫和扇棘单睾吸虫ITS2序列测定及其种系发育分析

doi:10.16431/j.cnki.1671-7236.2015.08.004

Abstract

Abstract: The morphological features of Haplorchis pumilio (H.pumilio) and H.taichui were observed with hydrochloric acid carmine staining; The ITS2 sequences were amplified by PCR, analyzed by sequencing, and compared with sequences of H.yokogawai and Metagonimus yokogawai of Heterophyidae trematodes downloaded from GenBank; And the phylogenetic tree was constructed by Mega 6.05.The morphological characteristics of H.pumilio and H.taichui from dog and cat were conformed to the classical taxonomic description.The ITS2 sequences of H.pumilio and H.taichui were 295 and 297 bp, and the percentages of G+C content of ITS2 were 49.5% (146/295) and 54.2% (161/297), respectively.The sequences were submitted to GenBank and the accession numbers of H.pumilio were KJ137221-KJ137223 and KP165437-KP165439, the accession number of H.pumilio was KP165440.The sequence alignment results showed that H.pumilio and H.taichui intraspecific difference was 0, interspecific differernce with four heterophyes was 15.2% to 28.9%.The phylogenetic tree based on ITS2 showed that H.pumilio and H.taichui were at the same clade with the bootstrap value of 99%.

Key words: Haplorchis pumilio; Haplorchis taichui; ITS2 gene; phylogenetic analysis

CLC Number:

MEI Xue-fang, LI Shu-qing, HU Chang-hong, HUANG Teng-fei, CHEN Zhi-fei, HUANG Wei-yi. ITS2 Sequencing and Phylogenetic Analysis of Haplorchis pumilio and H.taichui[J]. , 2015, 42(8): 1943-1949.

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ITS2 Sequencing and Phylogenetic Analysis of Haplorchis pumilio and H.taichui

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