猪繁殖与呼吸综合征病毒贵州PT分离株Nsp2基因克隆及序列变异分析

Abstract

Abstract: In order to know the gene sequence characteristics of porcine reproductive and respiratory syndrome virus (PRRSV) Guizhou PT isolate,a pair of specific primers of Nsp2 gene was designed according to the gene sequence of PRRSV JXA1 strain. The specific fragment of PRRSV Guizhou PT isolate was amplified by RT-PCR and sequenced after being cloned directly into pMD18-T. The softwares of DNAStar,PSORTⅡ,TMHMM and MLRC were used to predict the physical and chemical properties,homology,hydrophilicity,surface probability plot,antigenic index,secondary structure,subcellular localization. The results showed that the cDNA of Nsp2 was 588 bp;the predicted molecular weight was 21.5674 ku and pI was 5.02;it was an unstable protein. The study showed that Nsp2 possessed potential antigenicity and it mainly existed in the nucleus. It shared 80.0% to 99.8% of nucleotide homology with the 18 strains logged in GenBank. The conclusion showed that Nsp2 protein might have good antigenic characteristics,and genetic variation of Nsp2 gene was constantly emerging.

Key words: porcine reproductive and respiratory syndrome virus; Nsp2 gene; clone; sequence analysis

CLC Number:

ZHOU Si-xuan, XU Jian, WU Yu-tong, SHI Kai-zhi, XU Hong-zhon, XIAO Fang-ping. Cloning and Sequence Analysis of Nsp2 Gene of Porcine Reproductive and Respiratory Syndrome Virus Guizhou PT Isolate[J]. , 2014, 41(10): 38-42.

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Cloning and Sequence Analysis of Nsp2 Gene of Porcine Reproductive and Respiratory Syndrome Virus Guizhou PT Isolate

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