Abstract: This experiment was conducted to study the best extender and the way on frozen semen of Xinjiang donkey. The Xinjiang donkey semen was collected by artificial vagina, and diluted with five different extenders. Then the better extender was selected as a control group, and was respectively added by 4%, 5%, 6%, 8% glycerol. Finally the best extender was selected as a control group, and was concentrated. The results show that ①The frozen-thawing semen motility and acrosomal integrity that was frozen by the 5th extender were significantly higher（P<0.05） than that of 1, 2, 3, 4; ②Add 6 percent glycerol on the 7th extender was better（P<0.05） than the addition of 4%, 5%, 8%; ③The frozen-thawing semen motility and acrosomal integrity that was frozen by the 7th concentrated extender were significantly higher（P<0.05） than others. The results indicated that it is useful that the semen was diluted with the 6% glucose, 2% sucrose, 6% glycerol extender, and was concentrated 10 minutes in 500 r/min before being frozen.

Key words: donkey semen; frozen; glycerol; centrifugation