鲤鱼γ-2β干扰素基因组DNA克隆及序列分析

Abstract

Abstract: The research aimed to carry out the cloning, identification and sequence analysis of genomic DNA of carp interferon γ-2β (IFNγ-2β). On the basis of the full-length cDNA of carp, we designed two pairs of primers in its bilateral untranslated regions and the exons to perform PCR that used the carp spleen genomic DNA as the template, the IFNγ-2β full-length genomic DNA sequence was obtained. The full-length genomic DNA was 2084 bp, the GenBank accession number was JX181981, the gene sequence analysis showed the evolution of conservative and contained 4 exons and 3 introns were like most of the other species, the number of bases of each exon closed to the mammalian exons correspondingly. The exon and intron splice sites were complied with GT-AG rules.

Key words: common carp; interferon γ-2β; cloning; sequence analysis

CLC Number:

Q785

CHEN Yi-long, SUN Zhen, JIA Sheng-mei, FENG Xiang-ru, WANG Wen-dong, ZHANG Jun-hui, YANG Zhen-guo, LU Qiang. Cloning and Sequence Analysis of Interferon γ-2β (IFN γ-2β) Genomic DNA in Common Carp (Cyprinus carpio L.)[J]. , 2013, 40(3): 54-58.

References

1 蒋卫亮,曹俊明,赵红霞,等.鱼类的干扰素系统及干扰素功能[J].现代渔业信息,2009,24(10):9～11.
2 Ank N, West H, Paludan S R. IFN-lambda: Novel antiviral cytokines[J]. J Interferon Cytokine Res,2006,26:373～379.
3 Furnes C, Seppola M, Robertsen B. Molecular characterisation and expression analysis of interferon gamma in Atlantic cod[J]. Fish Shellfish Immunol,2009,26:285～292.
4 Gilbert W, De Souza S, Long M. Origin of genes[J]. Proc Natl Acad Sci USA,1997,94:7698～7703.
5 Igawa D, Sakai M, Savan R. An unexpected discovery of two interferon gammalike genes along with interleukin (IL)-22 and -26 from teleost: IL-22 and -26 genes have been described for the first time outside mammals[J]. Mol Immunol, 2006, 43: 999～1009.
6 Milev-Milovanovic I, Long S, Wilson M, et al. Identification and expression analysis of interferon gamma genes in channel catfish[J]. Immunogenetics, 2006, 58(1):70～80.
7 Nielsen H, Engelbrecht J, Brunak S, et al. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites[J]. Protein Eng, 1997,10:1～6.
8 Pestka S, Krause C D, Walter M R. Interferons, interferon-like cytokines, and their receptors[J]. Immunol Rev, 2004, 202:8～32.
9 Perussia B. Lymphokine-activated killer cells, natural killer cells and cytokines[J]. Curr Opin Immunol,1991,3:49～55.
10 Robertsen B. The interferon system of teleost fish[J]. Fish Shellfish Immunol,2006,20:172～191.
11 Sad S M R, Mosmann T R. Cytokine-induced differentiation of precursor mouse CD8⁺T cells into cytotoxic CD8⁺T cells secreting Th1 or T2 cytokines[J]. Immunity, 1995, 2:271～279.
12 Zou J, Yoshiura Y, Dijkstra J M,et al. Identification of an interferon gamma homologue in Fugu, Takifugu rubripes[J]. Fish Shellfish Immunol, 2004, 17(4):403～409.
13 Zou J, Carrington A, Collet B,et al.Identification and bioactivities of IFN-gamma in rainbow trout Oncorhynchus mykiss: The first Th1-type cytokine characterized functionally in fish[J].J Immunol, 2005, 175(4):2484～2494.

[1]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[2]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[3]	ZHANG Yongjie, SUI Zhiyuan, ZHANG Zhishuai, WANG Chenguang, LI Xiaojun, XING Feng. Cloning and Bioinformatics Analysis of NPTX1 Gene and Its Tissues Expression at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1048-1058.
[4]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[5]	TANG Jingyu, DU Hanyu, MENG Chunchun, LIU Guangqing. Cloning,Bioinformatics Analysis and Subcellular Localization of Interferon-stimulated Gene 15 in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2843-2854.
[6]	QI Nannan, QIAN Yonghang, LUO Gang, SITU Xuqi, LIU Jiying. Cloning, Bioinformatics Analysis and Tissue Expression Location of Porcine MORC2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2431-2441.
[7]	ZHANG Hang, SHA Huiyang, LI Huawei, HUANG Liangzong, ZHAO Mengmeng. Cloning, Bioinformatics and Tissue Expression Analysis of TRIM3 Gene in Swine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2474-2483.
[8]	YAN Yelian, ZHANG Mengya, LIU Qiuchen, WANG Xin, XU Changzhi, ZONG Yanfeng, ZHU Zhihua, WU Sucheng, SONG Yu, LI Yunsheng, ZHANG Yunhai, CAO Zubing. Research Progress of Epigenetic Reprogramming in Mammalian Somatic Cell Nuclear Transfer Embryo Development [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2259-2269.
[9]	WANG Baobao, JIN Hang, XIAN Yuhan, FENG Hongsheng, GAO Fengshan. Cloning SLA-1 Gene from Porcine Kidney Epithelial Cells and Analyzing its Molecular Structure Characteristics [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1671-1678.
[10]	ZHANG Jihu, MI Renisa, WANG Zixuan, LI Lin, XING Feng, LIU Shudong. Cloning and Bioinformatics of ERα Gene and Its Expression of Ovary Tissue in Cele Black Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1679-1687.
[11]	SHEN Xiang, CHEN Linghui, YAO Jingjie, WANG Sanhu, BAI Yueyu, ZHANG Xiaojian. Cloning,Bioinformatics and Expression Analysis of Bovine CCR7 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1244-1252.
[12]	ZHANG Zhishuai, SUI Zhiyuan, ZHANG Jihu, LI Qingjin, XING Feng. Cloning of CNP Gene and Its Tissue Expression Characteristics at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1402-1412.
[13]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[14]	HOU Jinxing, WANG Zhanhang, ZHU Junru, JIANG Yue, LIU Shujuan, AN Xiaopeng. Cloning, Bioinformatics and Tissue Expression Profile Analysis of CSN1S1 Gene in Guanzhong Dairy Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 857-865.
[15]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.

Cloning and Sequence Analysis of Interferon γ-2β (IFN γ-2β) Genomic DNA in Common Carp (Cyprinus carpio L.)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments