内毒素对肉鸡肝脏线粒体复活体Ⅰ活性的影响及氨基胍的保护效应

Abstract

Abstract: A total of 90 30-day-old commercial broilers were divided randomly into three groups that were respectively treated with LPS (lipopolysaccharide from Salmonella typhimurium, 5 mg/kg), AG (aminoguanidine, 300 mg/kg)+LPS and saline (2 mL/kg). After the injection of drugs, livers were taken from 6 killed chicken per group at five time points, i.e. 1, 3, 5, 7 and 9 h. At every time point, the mitochondria were determined for the evaluations of the concentrations of complexⅠ. The results indicated that LPS decreased significantly the activity of complexⅠof mitochondria in liver in broilers from 1 to 9 h(P<0.05). After the injections of AG, at 1 h, AG didn’t change the activity of complexⅠ(P>0.05), and increased significantly the activity of complexⅠat 3 h(P<0.05), and didn’t change the activity of complexⅠfrom 5 to 7 h(P>0.05), and decreased significantly the activity of complexⅠat 9 h(P<0.05),of the mitochondria in liver in broilers. It might be concluded that the LPS induced the damnification of mitochondria, resulted the disfunction of energy metabolism, decreased the production of ATP energy, resulted the impediment of activity of complexⅠ. AG played an important role in the objection of LPS and the protection of broilers.

Key words: broilers; endotoxin; lipopolysaccharide; aminoguanidine; mitochondria; complexⅠ

CLC Number:

Q591.4

SHI Dong-mei, WANG Yan, XIANG Rui-ping, HUANG PU He-ping, ZHANG Hua, TANG Zhao-xin, XU Jia-hua, WANG You-ling, ZHANG Sheng. Effect of Endotoxemia on Activity of ComplexⅠof Mitochondia in Liver and Protection of Aminoguanidine in Broilers[J]. , 2013, 40(1): 122-124.

References

1 王雪欣.抗生素诱导内毒素释放的研究进展[J].国外医药抗生素分册,2002,23(4):170~172.
2 刘林娜,董颖,李吉平,等. 蓖麻毒素诱导的小鼠淋巴细胞的凋亡[J].中国畜牧兽医, 2009, 36(2):46~49.
3 刘春峰,李巍,袁壮,等.SIRS大鼠心肌葡萄糖-6-磷酸酶活性的测定和线粒体形态变化[J].中国危重病急救医学,2001,1(6):147~149.
4 孟宪均.肝脏在多脏器功能衰竭发病中的作用[J]. 中华医学杂志,1988, 68(4):191~193.
5 赵永亮.内毒素血症特异抗体治疗[J].国外医学临床生物化学与检测分册,2002,23(3):170~172.
6 涂健,王艳萍,彭开松,等.内毒素诱发鸡多脏器损伤及其对肝TLR4 mRNA表达影响[J]. 生物学杂志,2007,24(6):13~15.
7 高洪,谭丽勤,李卫真,等.内毒素对山羊肝线粒体膜磷脂代谢及膜流动性的影响[J]. 中国病理生理杂志,2000,16(1):39~41.
8 夏晨梅,张顺财.内毒素血症致肝脏损伤[J].肝脏,2008,13(2):162~163.
9 黄永坤.胃肠黏膜屏障功能障碍及肠道细菌或内毒素移位对机体的影响[J]. 临床儿科杂志,2010,28(19):908~911.
10 梁锦屏,王琳琳,周娅.内毒素致肝损伤小鼠模型相关指标的动态变化[J]. 宁夏医学杂志,2007,29(8):678~680.
11 臧莹安,丁发源,向瑞平,等.从动物组织中提取线粒体的方法[J].中国兽医杂志,2006,42(2):61~62.
12 Cawthon D, Bottje W.Locaized defects in the electron transport chain(ETC) and alterations of pronton leak may underlie ascites-associated mitochondrial dysfunction in broilers[J].Poultry Science,1999,78(suppl.1):30.
13 Markley M A,Pierro A,Eaton S.Hepatocyte mitochondrial metabolism is inhibited in neonatal rat endotoxaemia: Effects of glutamine[J].Clinical Science(London),2002,102(3):337~344.
14 Tang Z, Iqbal M, Cawthon D, et al.Heart and breast muscle mitochondrial dysfunction in pulmonary hypertension syndrome in broilders[J].Comparative Biochemiastry and Physiology part A,2002,132(3):527~540.
15 Yi J H, Ni R Y, Luo D D, et al. Intestinal flora translocation and overgrowth in upper gastrointestinal tract induced by hepatic failure[J].World J Gast Roenterol, 1999, 5(4):327~329.

[1]	ZHONG Yueyun, YANG Shuzhan, CHEN Feiyue, LU Zhier, LI Bingxin, LI Wanyan, TIAN Yunbo, HUANG Yunmao, XU Danning, CAO Nan. Effects of LPS on Apoptosis of Goose Embryo-derived Hepatocytes ROS/p38/MAPK Signaling Pathway [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2224-2232.
[2]	WU Liting, LU Rui, LIU Banhong, BAO Hongduo, WANG Yongjuan, ZHOU Yan, WANG Ran, ZHANG Hui. Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2321-2332.
[3]	QIAO Hongxing, ZHANG Han, ZHAO Shengzhen, SUN Yanting, ZHANG Xiaojing, KANG Yu, BIAN Chuanzhou. Effects of Fermented Eucommia ulmoides Leaves on Production Performance, Antioxidant Properties and Immune Related Gene Expression in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1845-1854.
[4]	LI Mingguo, LI Qingchun, CHEN Xin, LU Shihao, HE Fan, QI Mengfan, ZHANG Huapeng, REN Yujun, ZHANG Qingze, FU Binbin, XU Mengsi, AI Zikai, YAN Kun, FENG Yun, HUA Zaidong, HUANG Tao, BI Yanzhen. Effects of TET Demethylase on Porcine Oocyte Development [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1907-1917.
[5]	SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.
[6]	LI Mengyuan, LIU Mengqian, KANG Jiajia, HUANG Ying, DUAN Yong, JIA Junjing, GE Changrong, CAO Weina. Effects of Guanidinoacetic Acid on Meat Quality and Abdominal Fat Deposition in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1385-1394.
[7]	ZHENG Xiaohui, LIU Kun, XIN Hangkuo, XIE Qingqing, ZHU Ting. Advance in the Regulation of Mitophagy in Neurodegenerative Diseases [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 490-499.
[8]	LI Bin, SHI Zhengling, ZENG Ziyou, HUANG Yingyan, LI Lin, DU Zilai, JI Yifan, WANG Di, YU Jie, MEI Shaofeng. Effects of Chicory on Growth Performance,Slaughter Traits and Meat Quality of Mahuang Broilers with Different Gender [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 500-511.
[9]	FU Jingcheng, HE Junhui, TONG Jiang, WU Wei, GUO Shuang, YANG Yanbin, HAN Yingqian, WANG Yueying, LI Heping. Anti-inflammatory and Antioxidant Roles of HO-1 in Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 807-816.
[10]	LIN Xiajing, ZHENG Chuntian, XI Pengbin, CHENG Zhonggang, DING Fayuan, RUAN Dong, WANG Yibing, JIANG Shouqun. Dietary Threonine Requirement of Yellow-feathered Broilers Aged From 22 to 42 Days [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 134-142.
[11]	WU Yanan, SHI Yuxiang, ZHANG Yongying, LIU Guanhui, SONG Weiguang, ZHONG Cuihong, WANG Yongxia. Study on Nrf2 Activation Alleviating Heat Stress-Induced Oxidative Damage in Broiler Cardiomyocytes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3343-3352.
[12]	GAO Jiahao, QIAO Yanjie, LIAN Kexun, WANG Mengmeng, GU Xinli, SHAO Yongbin. Effects of Glycyrrhizic Acid on Immune Performance of Lipopolysaccharide-stressed Yellow Feather Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3419-3427.
[13]	PENG Caichun, LI Binqiang, WANG Yeying, SU Haijun. Genetic Diversity Analysis of Sus scrofa Population in Guizhou Based on Mitochondrial CytbGene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2601-2612.
[14]	TANG Lin, ZHANG Junfang, WANG Ying, SUN Bin, WANG Enze, SHIN Jongsuh, GUO Panpan, JIN Xin, YAN Changguo, LI Xiangzi, LI Qiang. Anti-apoptotic Effect of Canine Adipose-derived Mesenchymal Stem Cells on Endoplasmic Reticulum Stress Model of Severe Acute Pancreatitis in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1840-1851.
[15]	LI Peiqi, KANG Jie, WANG Keyao, WANG Huimin, HAN Yufeng, WANG Tinghui, CUI Liang, ZHANG Jian, DENG Ruiqiang, DUAN Zhibian. Effect of Compound Traditional Chinese Medicine Water Extract on TGF-β1/Smad Pathway in Liver of Ascites Syndrom in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1860-1869.

Effect of Endotoxemia on Activity of ComplexⅠof Mitochondia in Liver and Protection of Aminoguanidine in Broilers

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments