内毒素对肉鸡肝脏线粒体复活体Ⅰ活性的影响及氨基胍的保护效应

Abstract

Abstract: A total of 90 30-day-old commercial broilers were divided randomly into three groups that were respectively treated with LPS (lipopolysaccharide from Salmonella typhimurium, 5 mg/kg), AG (aminoguanidine, 300 mg/kg)+LPS and saline (2 mL/kg). After the injection of drugs, livers were taken from 6 killed chicken per group at five time points, i.e. 1, 3, 5, 7 and 9 h. At every time point, the mitochondria were determined for the evaluations of the concentrations of complexⅠ. The results indicated that LPS decreased significantly the activity of complexⅠof mitochondria in liver in broilers from 1 to 9 h(P<0.05). After the injections of AG, at 1 h, AG didn’t change the activity of complexⅠ(P>0.05), and increased significantly the activity of complexⅠat 3 h(P<0.05), and didn’t change the activity of complexⅠfrom 5 to 7 h(P>0.05), and decreased significantly the activity of complexⅠat 9 h(P<0.05),of the mitochondria in liver in broilers. It might be concluded that the LPS induced the damnification of mitochondria, resulted the disfunction of energy metabolism, decreased the production of ATP energy, resulted the impediment of activity of complexⅠ. AG played an important role in the objection of LPS and the protection of broilers.

Key words: broilers; endotoxin; lipopolysaccharide; aminoguanidine; mitochondria; complexⅠ

CLC Number:

Q591.4

SHI Dong-mei, WANG Yan, XIANG Rui-ping, HUANG PU He-ping, ZHANG Hua, TANG Zhao-xin, XU Jia-hua, WANG You-ling, ZHANG Sheng. Effect of Endotoxemia on Activity of ComplexⅠof Mitochondia in Liver and Protection of Aminoguanidine in Broilers[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(1): 122-124.

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Effect of Endotoxemia on Activity of ComplexⅠof Mitochondia in Liver and Protection of Aminoguanidine in Broilers

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