鸡源益生菌的分离鉴定及其益生特性分析

doi:10.16431/j.cnki.1671-7236.2025.04.037

Abstract

Abstract: 【Objective】 The purpose of this experiment was to study the bacteriostatic effect of chicken derived Lactobacillus salivarius on Escherichia coli (CVCC1450) standard strain,and provide probiotic strains for the prevention and treatment of chicken colibacillosis in the future.【Method】 In this study,selective medium combined with molecular biology method was used to isolate probiotic strains from cloaca of healthy White laying hens.Morphological observation and 16S rDNA sequencing were used to identify the isolates,and the antibacterial activity and antibacterial substances of the isolates were analyzed.The growth curve and acid production curve of the isolates were established to explore their tolerance in the digestive tract,and their safety was evaluated by drug sensitivity test and hemolysis test.【Result】 Two strains of probiotics were isolated,all of which were Lactobacillus salivarius,and had good inhibitory effect on Escherichia coli with a diameter of more than 20 mm.After pH of the supernatant was adjusted to 7.0,the antibacterial activity of the isolates decreased significantly,indicating that the main antibacterial substance of the two strains of Lactobacillus salivarius was organic acid.Two strains of Lactobacillus salivarius had good growth characteristics and acid production ability.After 10 h of cultivation,they reached their plateau phase,and after 22 h of cultivation,the pH decreased to 3.75.The tolerance to bile salt solution and artificial gastrointestinal fluid of the two strains was strong,with a survival rate of over 27% in 0.3% bile salt solution and over 55% in artificial gastrointestinal fluid.Two strains of Lactobacillus salivarius were safe and sensitive to most antibiotics such as streptomycin,ampicillin,cefotaxime,ofloxacin,penicillin,cefazolin and doxycycline,and resistant to three antibiotics gentamicin,kanamycin and furazolidone,without hemolytic activity.【Conclusion】 In this study,two strains of Lactobacillus salivarus were isolated,which had good antibacterial activity against Escherichia coli,and laid a foundation for providing safe,efficient and non-residual green microecological preparations for the prevention and treatment of chicken and other poultry diarrhea caused by Escherichia coli.

Key words: Lactobacillus salivarius; biological characteristics; E.coli; antibacterial effect

CLC Number:

GUO Qing, WANG Hongli, FENG Yuxuan, ZHANG Yumeng, MA Qiyin, CHENG Jia, LIU Mingchao. Isolation and Identification of Chicken Probiotics and Analysis of Their Probiotic Properties[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1852-1861.

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Isolation and Identification of Chicken Probiotics and Analysis of Their Probiotic Properties

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