乙酰氨基阿维菌素混合胶束的制备、表征及体外透皮性能研究

doi:10.16431/j.cnki.1671-7236.2025.04.038

Abstract

Abstract: 【Objective】 The aim of this study was to prepare a kind of eprinomectin mixed micelle (EPR-MMs) which could be used for transdermal adminstration.【Method】 EPR-MMs were prepared by membrane hydration method using PEG-40 hydrogenated castor oi and nonyl phenol polyoxyethylene ether 40 as carriers.The particle size,polydispersity index (PDI),encapsulation efficiency,loading efficiency,and stability of EPR-MMs were evaluated through single-factor examination.Parameters such as the surfactant ratio,drug surfactant ratio,hydration rotation speed,and hydration time were screened and optimized to establish the best prescription and process parameters.The critical micelle concentration (CMC) was determined using the pyrene fluorescence probe method.The microstructure was characterized by transmission electron microscopy and Fourier transform infrared spectroscopy (FT-IR).The in vitro transdermal properties of EPR-MMs were investigated by using Franz diffusion cell,with a commercial pour-on agent as the control.【Result】 The optimum process prescription of EPR-MMs was as follows:Surfactant ratio was 20∶1,drug and surfactant ratio was 1∶9,hydration speed was 1 200 r/min and hydration time was 4 h.The CMC of EPR-MMs prepared under the optimal screening conditions was 11.596 μg/mL.EPR-MMs was spheroid under transmission electron microscope,with suitable particle size and good morphology.FT-IR analysis confirmed that the drug was encapsulated in the micelle.Stability studies showed that the prepared EPR-MMs had good stability when placed at room temperature for 7 d.In vitro permeation results demonstrated that the cumulative permeation amount per unit area of EPR-MMs was 44.26 μg/cm²,which was about 3.82 times that of the pour-on,and the penetration rate was 3.90 times that of the pour-on. 【Conclusion】 The stable EPR-MMs with suitable particle size was successfully prepared,and its physicochemical properties were verified by multiple characterization methods,showing good in vitro release characteristics,and higher transdermal rate and permeability than the commercial pour-on.

Key words: eprinomectin (EPR); mixed micelles; transdermal adminstration

CLC Number:

S859.79⁺5

MAO Yujuan, ZHANG Hongxiu, CHEN Wei, YU Shenglan, GUO Liuna, GAO Jie. Preparation,Characterization and in vitro Transdermal Properties of Eprinomectin Mixed Micelles[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1862-1872.

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Preparation,Characterization and in vitro Transdermal Properties of Eprinomectin Mixed Micelles

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