犏牛主要组织相容性复合物DRA基因 (MHC-ClassⅡ-DRA)的分子特征分析

Abstract

Abstract: To explore the resistance traits of Chinese Yakow, in the present study, the Yakow coding region sequence of the DRA gene had been amplified by one pair primer and deposited into the GenBank database under accession JQ347519. According to the ORF FINDER from NCBI and other bioinformatics tools, the length of the coding region was 762 bp which encoded 253 amino acids. Moreover, the characteristics of hydrophobicity, signal peptide, N-linked glycosylation and the secondary structure were predicted. Analysis the homology with other species and compared with cattle, Yakow DRA (bogr×bola-DRA) sequence showed highest similarity (99.0%). Unlike other MHC molecules, the DRA gene showed highly conserved characteristics across all animals especially ruminants in functional positions, such as antigen binding at peptide binding site (PBS), N-linked glycosylation, α1 and α2 domains.

Key words: Chinese Yakow; MHC; DRA; resistance

CLC Number:

PU Lan-ping, XI Dong-mei, FAN Yue-yuan, SU Ba-qi, CHEN Xue-li, LI Ji-zhong, DENG Wei-dong. Analysis of the Molecular Characteristics of Chinese Yakow Major Histocompatibility Complex DRA Gene(MHC-ClassⅡ-DRA)[J]. .

References

1 禹文海, 鲁绍雄. 牛MHC(BOLA)基因的研究进展[J].中国牛业科学, 2007, 33(1):21~23.

2 徐飞, 成述儒, 罗玉柱. 奶牛DRB3基因生物信息学分析[J].中国奶牛, 2011, 2:4~7.

3 高晓明. 免疫学教程[M].北京:高等教育出版社,2006.

4 廖贤军, 赖松家. BOLA在牛抗病育种中的研究进展[J].中国畜牧兽医, 2006, 33(1):33~35.

5 Aarestrup F M, Jensen N E, Ostergärd H. Analysis of associations between major histocompatibility complex(BOLA)class haplotypes and subclinical mastitis of dairy cows[J]. J Dairy Sci, 1995, 78:1684~1692.

6 Aida Y, Kohda C, Morooka A, et al. Cloning of Cdna and the molecular evolution of bovine MHC class Ⅱ DRA gene[J]. Biochem Biophy Res Comm, 1994, 204:195~202.

7 Alizadeh Z, Karrow N, Mallard B A,et al. Biological effect of varying peptide binding affinity to the BOLA-DRB3*2703 allele[J]. Genet Sel Evol, 2000, 35(1): 51~65.

8 Berryrer T G, Carruthers T D, Rabbins J W, et al. Resistance to staphylococcus aureus mastitis detected by DNA marker. Proc 31^st Annu Meet Natl Mastitis Counc,1992.

9 Larruskain A, Minguijn E, García-Etxebarria K, et al. MHC class Ⅱ DRB1 gene polymorphism in the pathogenesis of Maedi-Visna and pulmonary adenocarcinoma viral disease in sheep[J]. Immunogenetics, 2010, 62(2):75~83.

10 Maillard J C, Berthier D, Chantal I, et al. Selection assisted by a BOLA-DR/DQ haplotype against susceptibility to bovine dermatophilosis[J]. Genet Sel Evol, 2003, 35(1): 193~200.

11 Mirsky M L, Olmstead C, Da Y, et al. Reduced bovine leukaemia virus proviral load in genetically resistant cattle[J]. Anim Genet, 1998, 29(4):245~252.

12 Sakaram D, Niranjan S K, Subodh K, et al, cDNA characterization and molecular analysis of buffalo MHC class Ⅱ gene, DRA(Bubu-DRA)[J]. J Appl Anim Res, 2010, 37: 118~121.

13 Sena L, Schneider M P C, Brenig B, et al. Polymorphism in MHC DRA and DRB alleles of water buffalo (Bubalus bubalis) reveal different features from cattle DR alleles[J]. Anim Genet, 2003, 34:1~10.

14 Sharif S, Mallard B A, Wilkie B N, et al. Associations of the bovine major histocompatibility complex DRB3 (BoLA-DRB3) alleles with occurrence of disease and milk somatic cell score in Canadian dairy cattle[J]. Anim Genet, 1998, 29:185~193.

15 van Eijk M J, Stewart-Haynes J A, Beever J E, et al. Development of persistent lymphocytosis in cattle is closely associated with DRB2[J]. Immunogenetics, 1992, 37(1):64~68.

16 Walter J, High K A. Gene therapy for the hemophiliac[J]. Adv Vet Med, 1997, 23(12):119~134.

17 Yang J, Huston L, Berger D, et al. Expression of HLA-DP0401 molecules for identification of DP0401 restricted antigen specific T cells[J]. J Clin Immunol, 2005, 25:428~436.

18 Zanotti M, Poli G, Ponti W, et al. Association of BOLA class Ⅱ haplotypes with subclinical progression of bovine leukaemia virus infection in Holstein-Friesian cattle[J]. Anim Genet, 1996, 27(5):337~341.

[1]	CHEN Jianshi, ZHANG Ning, PU Fangxiong, MIAO Jianjia, ZENG Nan, LI Bingxue. Prediction of Bacillus anthracis Carrying Prophages and Analysis of Drug Resistance and Virulence [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2573-2584.
[2]	XIE Hualiang, LEI Xiaowen, LI Jianjun, LIU Zhenni, ZHONG Yunping, TAN Donghai, ZHANG Qiang. Effects of Earthworm Hydrolysate on Laying Performance,Serum Biochemical Indices,Antioxidant Indices and Immune-related Indices of Laying Hens Aged 105 to 175 Days [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1899-1906.
[3]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[4]	ZENG Chengrong, LIU Xin, BI Wenwen, MEI Shihui, HE Guangxia, ZHANG Junjie, WEN Ming, ZHOU Bijun, CHEN Jiangfeng, JIANG Haibo. Research Progress on Quorum Sensing System of Aeromonas hydrophila and Its Inhibitor Application [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1991-2000.
[5]	WANG Lucai, SALANG Wenzhu, ZHANG Huanrong. Isolation,Identification and Drug Resistance and Pathogenicity Analysis of Salmonella Enteritidis from Avian [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1663-1674.
[6]	WU Zhi, ZHANG Cong, YUAN Huisha, DONG Hongyan, DAI Lixia, JIA Luqi, WU Shuang, ZHU Shanyuan. Isolation,Identification,Virulence and Drug Resistance of Salmonella from Yellow Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1695-1706.
[7]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[8]	ZHU Qiqi, ZHANG Jiyu, WANG Chengye, WU Lingyu, WANG Weiwei, ZHU Zhen. Analysis of Multidrug Resistance and Prevalence of Animal-derived Escherichia coli in Hebei [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1081-1092.
[9]	ZHU Xihui, LI Yanlan, WANG Zhiwei, PANG Zheyu, LI Yubao, LIU Cheng, SI Zhenshu, LU Jianbiao, CAO Shengliang, XUE Xijuan. Whole Genome Sequencing of a Resistant Riemerella anatipestifer Strain and Analysis of Antibiotic Resistance and Genetic Evolution [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1140-1149.
[10]	HU Yifan, MA Jinghua, BAI Siqi, ZHAI Yimeng, LIU Jianxun, SONG Houhui, YANG Yongchun. Isolation,Identification and Biological Characteristics Analysis of Morganella morganii from Endometritis of South China Tiger (Panthera tigris amoyensis) [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1271-1278.
[11]	TIAN Erjie, GU Zhen, WANG Zihao, LIU Fangzheng, LIU Kesen, ZHANG Xuefei, ZHOU Bianhua. Study on the Drug-resistance Elimination of Scutellaria baicalensis on Apramycin-resistant Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1279-1286.
[12]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[13]	MA Xiaojiao, ZHAO Yankun, SHAO Wei, WU Yating, LI Ming, LIU Huimin, MENG Lu, CHEN He. Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus in Raw Milk in Some Areas of Xinjiang [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 789-797.
[14]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[15]	LIU Lin, WU Baoqing, GUO Xiaoyin, YU Wenhui, TIAN Lang. Study on Bacteriostatic Action of Aqueous Extract of Granati pericarpium Against Multi-drug Resistant Escherichia coli of Swine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 328-340.

Analysis of the Molecular Characteristics of Chinese Yakow Major Histocompatibility Complex DRA Gene(MHC-ClassⅡ-DRA)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments