兔防御素基因NP-1的克隆及其在大肠杆菌中的表达

China Animal Husbandry & Veterinary Medicine

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Artificial Synthesis of Rabbit Defensin NP-1 Gene and its Expression in E.coli

QIN Xiao-yu^1,2, PENG Zhang-hua³, DONG Xiao², MIAO Xiang-yang¹, LUO Qing-miao¹

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Qingdao Agricultural University, Qingdao 266109, China;
3. Sichuan Luxian Country Animal Husbandry Bureau, Luzhou 646100,China

Received:2012-03-13 Online:2012-09-20 Published:2012-09-18

Abstract

Abstract: The mature peptide sequence of rabbit defensin NP-1 gene was modified with favour codes for E.coli and synthesized chemically.The target gene fragment modified was cloned into vector T-easy,and then inserted into fusion expression plasmid pMAL-p2X through digesting of two restriction enzymes.The positive recombinant expression vector pMAL-NP-1 was transformed into E.coli TB1,which could effectively produce the fusion protein MBP and NP-1 induced with IPTG.

Key words: animal science; defensin; fusion protein; expression

CLC Number:

Q78

QIN Xiao-yu, PENG Zhang-hua, DONG Xiao, MIAO Xiang-yang, LUO Qing-miao. Artificial Synthesis of Rabbit Defensin NP-1 Gene and its Expression in E.coli[J]. China Animal Husbandry & Veterinary Medicine.

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Artificial Synthesis of Rabbit Defensin NP-1 Gene and its Expression in E.coli

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