不同消化酶对羊驼皮肤黑素细胞分离的影响

Abstract

Abstract: In order to explore and establish a new method of isolation,culture,and identification of alpaca skin melanocyte in vitro for further studying to provide a suitable pigment deposition cell model. Take 1 to 3 years young alpacal skin,with three different enzymes (trypsin,trypsin-EDTA,Dispase Ⅱ) digestion alpaca skin,alpaca melanocyte were obtained by trypsin-EDTA digestion,placed into DMEM/F12 culture medium. Analyze melanocyte growth,cell yields and cells attached.The results showed Dispase Ⅱ group had a significant higher other groups (P＜0.01).In summary,alpaca melanocyte were obtained by Dispase Ⅱ digestion.

Key words: alpaca; melanocyte; DispaseⅡ; depuration

CLC Number:

Q813.1

BAI Rui, YU Zhi-hui, YANG Gang, WANG Hai-dong, FAN Rui-wen, DONG Chang-sheng. Effects of Different Enzymes on the Melanocyte Isolation of Alpaca Skin[J]. .

[1]	ZHANG Min, ZHENG Wenxin, WANG Le, GAO Weiming, CHEN Huafeng, GONG Ping, HU Xin, GAO Yang, KUMUS·Habuduola, AYGL·Yusufu. Study on the Quality Traits of Alpaca Wool in Different Regions and Body Parts [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3602-3610.
[2]	XIONG Heli, XI Dongmei, LI Guozhi, WANG Liping, LIU Xiangying, YUAN Mengya, LI Jing, DENG Weidong. Research Advances on the Formation Mechanism of Animal Melanocytes and Its Related Traits [J]. , 2019, 46(1): 72-79.
[3]	XU Yanli, XING Weiting, LV Xuefeng, HU Xin, LUO Xin, WANG Le. Analysis of Alpaca Wool Quality in Akesu Area of Xinjiang [J]. , 2018, 45(6): 1639-1644.
[4]	LIU Ying, XUE Linli, CHANG Lucheng, BAI Yun, LI Lixin, LI Jingwei, YU Xiuju, HE Xiaoyan, WANG Haidong. Effects of EDN3 on the Expression of Melanin-related Genes in Mouse Melanocytes [J]. , 2018, 45(3): 581-589.
[5]	LV Xue-feng, XING Wei-ting, XU Yan-li, ZHANG Min, WANG Le. Genetic Diversity and Phylogenetic Relationship of Introduced Alpaca in Xinjiang [J]. , 2017, 44(9): 2709-2715.
[6]	XU Yan-li, HU Xin, XING Wei-ting, ZHANG Min, WANG Le. Correlation Analysis on the Fineness and Single Fiber Strength of Alpaca Wool Fiber [J]. , 2017, 44(10): 2980-2986.
[7]	WU Yue-hong, LI Yong, YANG Yi, HE Yu-long. Research Progress on Regulation of microRNAs in Animal Skin and Hair Follicle [J]. , 2016, 43(9): 2373-2378.
[8]	YANG Yu-jing, NIE Rui-qiang, XIE Jian-shan, FAN Rui-wen, DONG Chang-sheng. Cloning and Bioinformatics Analysis on CDS of Oct-1 Gene in Mouse [J]. , 2016, 43(8): 1960-1966.
[9]	YANG Chun-ling, HAO Tian-tian, LI Qiang-fei, CHEN Ting, MAO Hua-ming, DENG Wei-dong. Signal Regulation for Melanoblast Cytopoiesis Process：A Review [J]. , 2013, 40(9): 187-194.
[10]	GAO Rong-kun, LI Jian-ping, QU Hai-e, JIANG Qian, CHEN Wei, WANG Ying, ZHANG Qiao-ling. Cloning and Sequence Analysis of Huacuya Alpacas KIT Gene Exon2 [J]. , 2013, 40(8): 42-45.
[11]	ZHANG Dan-li, TIAN Xue, SONG Yun-fei, MU Ren, DONG Chang-sheng. Expression of miR-193b and its Target Gene Kit in Different Colors of Alpaca Skin [J]. , 2013, (5): 44-49.

Effects of Different Enzymes on the Melanocyte Isolation of Alpaca Skin

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics

Comments