抗缪勒氏管激素抑制山麻鸭卵泡发育分子机制的研究

doi:10.16431/j.cnki.1671-7236.2023.08.021

Abstract

Abstract: 【Objective】 Investigating the inhibition of anti-Müllerian hormone (AMH) on follicular development and the molecular mechanism in Shan-ma ducks.【Method】 40 healthy 29-week-old Shan-ma ducks were randomly divided into 4 groups.AMH1,AMH2 and AMH3 groups were given 2,20 and 200 μg/d AMH for 21 d,respectively.And the control group was given saline.During the experiment,egg production was recorded daily.Samples were collected at the end of this experiment,the number of follicles were counted,and calculated the gonadosomatic index (GSI).Serum luteinizing hormone (LH),progesterone (P₄) and estradiol (E₂) levels,the gene expression levels of Follicle stimulating hormone receptor (FSHR),luteinizing hormone receptor (LHR),steroidogenic acute regulatory protein (StAR),3β-hydroxysteroid dehydrogenase (3β-HSD),estrogen synthase (CYP19A1),growth differentiation factor 9 (GDF9),bone morphogenetic protein 15 (BMP15) in graded follicles (F6),small yellow follicles (SYF) and large white follicles (LWF),and the protein levels of StAR and GDF9 in F6 and SYF were detected.【Result】 Compared with control group, the laying rate were significantly decreased in AMH2 and AMH3 groups (P＜0.05),and AMH3 group showed the most significant inhibition of egg production rate. The numbers of SYF and LWF were significantly increased in AMH1 and AMH2 groups (P＜0.05), while GSI and the numbers of LYF had no significant trend. Serum LH, P4 and E2 level were in dose-dependent reduction, and AMH3 group showed a more pronounced inhibition of these hormones. LH level was significantly decreased in AMH3 group (P＜0.05). Compared with control group, the expression of GDF9 gene in AMH2 group and BMP15 gene in AMH1 group were significantly increased in F6 (P＜0.05). In SYF, except for GDF9 gene in the AMH2 group, the expression of FSHR gene was significantly decreased, while the expression of GDF9 and BMP15 genes in each experiment group were significantly increased (P＜0.05). The GDF9 protein level in AMH3 group was significantly increased (P＜0.05). The expression of GDF9 and BMP15 genes in AMH2 and AMH3 groups were significantly increased in LWF (P＜0.05). The administration of exogenous AMH did not significantly affect the expression of CYP19A1,3β-HSD and StAR genes in F6 and LWF (P＞0.05). In SYF, CYP19A1,3β-HSD and StAR genes were observed in AMH1, AMH2, and AMH3 groups were significantly lower than those of control group (except for CYP19A1 gene in AMH2 group, P＜0.05). Compared with control group, the administration of exogenous AMH had no significant effect on the protein levels of StAR in F6 and SYF (P＞0.05).【Conclusion】 200 μg/d exogenous AMH could significantly inhibit follicular development,mainly by inhibiting the secretion of LH,P₄,and E₂,upregulating the expression of GDF9 and BMP15 in follicles,downregulating the expression of FSHR and LHR,as well as key factors affecting steroid hormone synthesis pathways.

Key words: anti-Mullerian hormone(AMH); gonadotropin receptor; steroid hormone; follicular development; Shan-ma ducks

CLC Number:

S859

ZHOU Xiaoli, ZHANG Zhuoshen, SHEN Xu, PAN Jianqiu, HUANG Yunmao, JIANG Danli. Study on the Molecular Mechanism of Inhibition of Follicular Development in Shan-ma Ducks by Anti-Müllerian Hormone[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3239-3247.

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Study on the Molecular Mechanism of Inhibition of Follicular Development in Shan-ma Ducks by Anti-Müllerian Hormone

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