1株植物乳杆菌的生物学特性及体外抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2023.02.024

Abstract

Abstract: 【Objective】 The purpose of this study was to investigate the biological characteristics and antibacterial activity in vitro of a Lactobacillus plantarum GX20200417-1,and discuss the possibility of its application in production practice.【Method】 The GX20200417-1 strain was inoculated into PBS with different pH and different concentrations of bile salt,and artificial gastrointestinal juice,its tolerance to acid,bile salt and artificial gastrointestinal juice were analyzed by colony count. The antibacterial activities of Lactobacillus plantarum GX20200417-1 strain against Escherichia coli, Staphylococcus aureus and Salmonella were detected by Oxford cup method.The degradation ability of Lactobacillus plantarum to mycotoxin was studied by ELISA method after co-culture with mycotoxin.Finally,the mice were fed for safety test.【Result】 Lactobacillus plantarum GX20200417-1 strain could tolerate pH 2.0 and 0.3% bile salt,and had at least 1×10⁴CFU/mL viable bacteria in artificial gastrointestinal juice.The supernatant of Lactobacillus plantarum culture had obvious inhibitory effect on Escherichia coli, Salmonella and Staphylococcus aureus,it could degradation gibberellin and aflatoxin,but it has no effect on the degradation of vomiting toxin.The mice were safe without side effects after Lactobacillus plantarum GX20200417-1 strain gavage.【Conclusion】 Lactobacillus plantarum GX20200417-1 strain had excellent probiotic properties,and had certain development potential in livestock and poultry production.

Key words: Lactobacillus plantarum; GX20200417-1 strain; biological characteristics; antibacterial activityin vitro

CLC Number:

S816.3

YIN Yangyan, PAN Yan, LI Xiaoning, LI Jun, LIAO Yuying, LI Changting, WEI Tianchao, PENG Hao. Study on Biological Characteristics and Antibacterial Activity in vitro of a Lactobacillus plantarum Strain[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 666-673.

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Study on Biological Characteristics and Antibacterial Activity in vitro of a Lactobacillus plantarum Strain

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