京津冀部分地区致犊牛腹泻大肠杆菌毒力基因、耐药基因检测及药物敏感性分析

doi:10.16431/j.cnki.1671-7236.2022.12.030

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the prevalence of virulence genes and drug resistance genes of Escherichia coli (E.coli) isolated from diarrheic calves in Beijing-Tianjin-Hebei regions, providing basis for the screening of sensitive drugs.【Method】 In this study, 146 fecal samples of diarrheic calves were collected from different cattle farms in Beijing-Tianjin-Hebei regions from December 2020 to July 2021. E.coli was isolated, purified and identified by Gram staining microscopy and 16S rRNA sequencing.The virulence genes (F17, K99, F41, STa, stx1, irp2 and fyuA genes) and drug resistance genes (aac(6')-Ⅰb, bla_CTX-M, bla_TEM, OqxB, tetA and sul1 genes) were identified by PCR.The drug sensitivity test was performed using K-B paper method.【Result】 The growth morphology of the isolates on the medium and the gram staining results was consistent with the physiological and biochemical characteristics of E.coli.The 16S rRNA sequencing results of the isolates showed a single peak, and more than 96% of the sequences were identical to E.coli genome using NCBI BLAST, indicating the clinical isolates were E.coli.A total of 142 strains of E.coli were isolated, of which 88 strains carried virulence genes, accounting for 61.97% (88/142).The positive rates of virulence genes F17, K99, F41, STa, stx1, irp2 and fyuA were 24.65%, 0.70%, 0, 2.11%, 1.41%, 45.07% and 21.83%, respectively.Therefore, F17, irp2 and fyuA were the dominant virulence factors, while E.coli carrying multiple virulence factors was less frequently detected.Six resistance genes aac(6')-Ⅰb, bla_CTX-M, bla_TEM, OqxB, tetA and sul1 were detected.bla_TEM gene had the highest detection rate of 45.77%.In addition, aac(6')-Ⅰb and OqxB genes had the lowest detection rate of 9.15%, and the isolated strains mainly carried 1 to 3 resistance genes.The results of drug susceptibility test on 142 isolates showed the highest susceptibility rate of norfloxacin, followed by ciprofloxacin.The susceptibility rate to penicillin was 0, and the drug resistance phenomenon was severe, with 86.62% of isolates resistant to more than 2 antimicrobial agents.【Conclusion】 The virulence genes and drug resistance genes of E.coli isolated from diarrheic calves in Beijing-Tianjin-Hebei regions were widespread distributed and highly drug resistance.The phenomenon of multiple drug resistance was severe.This study provided a theoretical basis for the prevention and treatment of E.coli induced calf diarrhea in Beijing-Tianjin-Hebei regions.

Key words: calf diarrhea; Escherichia coli; virulence genes; drug resistance genes; drug resistance phenotype

CLC Number:

S852.61^＋2

JIA Tong, ZHANG Di, ZHANG Zhaotian, CHEN Panliang, ZHANG Jie, LI Yinghui, ZHU Yue, QIN Jianhua, LI Yan. Detection of Virulence and Resistance Genes and Drug Sensitivity Analysis of Escherichia coli Derived from Diarrheic Calves in Beijing-Tianjin-Hebei Partial Regions[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4820-4831.

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Detection of Virulence and Resistance Genes and Drug Sensitivity Analysis of Escherichia coli Derived from Diarrheic Calves in Beijing-Tianjin-Hebei Partial Regions

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