硫化氢对顺铂致犬肾脏氧化损伤及炎症的缓解作用

doi:10.16431/j.cnki.1671-7236.2022.06.037

Abstract

Abstract: 【Objective】 The aim of this study was to explore the effect of hydrogen sulfide (H₂S) on the acute kidney injury (AKI) of dog induced by cisplatin.【Method】 18 healthy adult Beagle dogs were randomly divided into three groups:Control group (C), cis group and H+cis group.The dogs in control group were injected with normal saline intravenously, the dogs in cis group were injected with 5 mg/kg cisplatin intravenously, the dogs in H+cis group were injected with 1 mg/kg NaHS intravenously, and after 30 min cisplatin was injected, NaHS solution was injected once every 24 h, a total of 3 times.The dogs were anesthetized 72 h after cisplatin injection, and blood was collected from the vein to test the serum creatinine (Scr) and urea nitrogen (BUN).The kidney pathological changes were observed by HE staining.The contents of glutathione (GSH), hydrogen peroxide (H₂O₂) and nitric oxide (NO) in kidney tissues of dog were detected by biochemical kits.The mRNA and protein expressions of IL-1β, IL-6, TNF-α, NF-κB, COX2, iNOS, IL-4 and IL-10 in kidney were detected by Real-time quantitative PCR and Western blotting.【Result】 The levels of Scr and BUN in cis group were extremely significantly higher than those in control group (P<0.01), and the levels of Scr and BUN in H+cis group were extremely significantly lower than those in cis group (P<0.01).The results of pathological examination showed that there were significant pathological changes in kidney of dogs in cis group, while the pathological changes in kidney of dogs of H+cis group were alleviated.The biochemical test results showed that compared with C group, the content of GSH in kidney of cis group was decreased extremely significantly (P<0.01), and the contents of H₂O₂ and NO were increased extremely significantly (P<0.01);Compared with cis group, the content of GSH in kidney of H+cis group was increased extremely significantly (P<0.01), while the contents of H₂O₂ and NO were decreased extremely significantly (P<0.01).The results of Real-time quantitative PCR and Western blotting showed that compared with C group, the mRNA and protein relative expressions of the proinflammatory factor IL-1β, IL-6, TNF-α, NF-κB and COX2 in cis group were increased significantly or extremely significantly (P<0.05;P<0.01), while the mRNA and protein relative expressions of anti-inflammatory factors IL-4 and IL-10 were decreased extremely significantly (P<0.01);Compared with cis group, the mRNA and protein expressions of anti-inflammatory factors IL-4 and IL-10 in kidney of dogs in H+cis group were extremely significantly higher (P<0.01), while the mRNA and protein relative expressions of pro-inflammatory factor IL-1β, IL-6, TNF-α, NF-κB and COX2 were decreased extremely significantly (P<0.01).【Conclusion】 H₂S could improve cisplatin induced AKI in dogs by enhancing the antioxidant capacity of kindey and inhibiting the expression of inflammatory factors.

Key words: dogs; hydrogen sulfide; acute kidney injury; inflammation; oxidative stress

CLC Number:

S852.2

WANG Shuang, LIU Xingyao, JIA Haotian, LIU Yun. Alleviative Effect of Hydrogen Sulfide on Cisplatin-induced Kidney Oxidative Damage and Inflammation in Dogs[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2376-2384.

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Alleviative Effect of Hydrogen Sulfide on Cisplatin-induced Kidney Oxidative Damage and Inflammation in Dogs

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