不同浓度芦丁和冷冻速率对猪精子冷冻保存效果的研究

doi:10.16431/j.cnki.1671-7236.2022.03.020

Abstract

Abstract: 【Objective】 The purpose of this study was to investigate the effects of different concentrations of rutin and different freezing rates on the cryopreservation of Duroc boar sperm and their interaction, in order to provide basis for guiding production practice and improving utilization rate of excellent breeding pigs.【Method】 The experiment was divided into 6 groups:Blank control group (frozen diluent solution Ⅰ), and experimental groups (0.2, 0.4, 0.6, 0.8 and 1.0 mmol/L rutin were added to the frozen diluent solution Ⅰ, respectively).Semen were frozen at 1 cm (fast freezing) and 3 cm (slow freezing) above the liquid nitrogen surface, respectively.After being stored in liquid nitrogen for 30 days, sperm motility parameters, membrane integrity rate (MI), acrosome integrity rate (AI), mitochondrial membrane potential(MMP), DNA integrity rate, ROS level, MDA and ATP content, and the activities of SOD, CAT and GSH-Px were measured to evaluate the frozen-thawed semen quality.【Result】 In terms of freezing rate, in the same concentration of rutin freezing solution, the slow freezing effect was better than the fast freezing effect, and the difference was significant (P<0.05).In terms of rutin concentration, the effect of adding 0.6 mmol/L rutin was the best (P<0.05), followed by 0.8 mmol/L.In terms of their interaction, there was an interaction between freezing rate and rutin concentration, especially sperm motility parameters, membrane integrity, acrosome integrity, DNA integrity, mitochondrial membrane potential and ATP content of slow freezing×0.6 mmol/L rutin combination were significantly higher than those of other combination experimental groups (P<0.05), and the level of ROS was significantly lower than that of other combination experimental groups(P<0.05).The activities of SOD, CAT and GSH-Px were significantly increased compared with other groups.【Conclusion】 There were interaction effects between different rutin concentrations and different freezing rates, and adding 0.6 mmol/L rutin into the slow freezing diluent had the best effect.

Key words: rutin; boar; freezing rate; semen quality; interaction effect

CLC Number:

S828

LUO Lan, CUI Chao, DONG Ruilan, LIU Haoyu, LIU Xiaobin, YU Guanghui. Effects of Different Concentrations of Rutin and Freezing Rates on Cryopreservation of Boar Semen[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 982-991.

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Effects of Different Concentrations of Rutin and Freezing Rates on Cryopreservation of Boar Semen

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