重组动物疱疹病毒活载体疫苗的构建与应用研究进展

doi:10.16431/j.cnki.1671-7236.2022.01.036

Abstract

Abstract: Recombinant virus live vector vaccines are live vector vaccines constructed through genetic engineering methods, avoiding some of the shortcomings of traditional vaccines, and have great advantages and application prospects. Animal Herpesviruses currently widely used as recombinant virus vectors include Herpesvirus of turkey (HVT), Pseudorabies virus (PRV), Duck enteritis virus (DEV), and avian Infectious laryngotracheitis virus (ILTV), etc. Analysis of the Herpesvirus genome sequence shows that its genome is large, there are many unnecessary regions for replication, and the ability to accommodate exogenous gene is strong. At the same time, the Herpesvirus has the advantages of wide host range, long duration of immunity, good safety and mature related gene manipulation technology. This article summarized the main methods of constructing recombinant animal Herpesviruses, including traditional homologous recombination technology, bacterial artificial chromosome technology (BAC), Fosmid library and CRISPR/Cas9 gene editing technology, and briefly described the basic principles and technical characteristics of each method, compared the advantages and disadvantages of each method, analyzed the optimal application conditions of each method, so as to provide more accurate theoretical and technical references for research work. The expression of exogenous genes were analyzed, and examples were given to demonstrate the different regulatory effects of different promoters on the expression of exogenous genes. At the same time, the common insertion sites of different Herpesviruses were listed and summarized. The research progress and the registration application information of related biological products of the current recombinant viruses HVT, PRV, DEV and ILTV live vector vaccines were summarized, and the high-selectivity exogenous genes and high-success rate insertion sites were induced, so as to provide a theoretical reference for the development of related biological products.

Key words: recombinant animal Herpesvirus; live vector vaccine; Herpesvirus of turkey (HVT); Pseudorabies virus (PRV); Duck enteritis virus (DEV); Infectious laryngotracheitis virus (ILTV)

CLC Number:

S852.65⁺9.1

YANG Mingzhu, CHEN Xiaochun, SONG Jiacheng, HOU Yaxin, LI Junping. Research Progress on Construction and Application of Recombinant Animal Herpesvirus Live Vector Vaccine[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 338-351.

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Research Progress on Construction and Application of Recombinant Animal Herpesvirus Live Vector Vaccine

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