病毒性动物疫病诊断技术研究进展

doi:10.16431/j.cnki.1671-7236.2022.01.032

Abstract

Abstract: The outbreak of animal epidemic diseases not only seriously affects the production and development of animal husbandry, causing economic losses, but also seriously endangers human health. The increase of viral diseases makes animal diseases more complex and the epidemic trend more severe. Sensitive, specific, rapid diagnosis of animal epidemic diseases is the key link to implement disease prevention and control measures. Molecular biological diagnosis based on PCR technology and isothermal amplification technology has become a commonly used pathogen screening method, because it solves the problems of traditional pathogen diagnosis, such as time-consuming and laborious, window period of immune serology, and gets rid of the dependence on high-precision temperature control equipment. However, most detection methods can only detect one or several pathogens at one time, and the complex test process and high requirements for facilities reduce the ability of immediate intervention in the outbreak, and cannot meet some detection and analysis needs. In recent years, interdisciplinary high-throughput diagnostic technology has developed rapidly. It can monitor, detect and characterize hundreds of targets at the same time, which is suitable for the rapid detection of pathogens in various environments. It is an important biological detection method in the development of life science. In this review, we summarized several major viral animal disease detection methods including etiology, immune serology, molecular biology, and high-throughput diagnosis. Looking forward to the development of animal disease diagnosis and detection technology in the future, and these methods provide more capability for the rapid diagnosis of animal diseases and improve the prevention and control ability of animal diseases.

Key words: virus; animal epidemic diseases; diagnosis technologies

CLC Number:

S855.3

YANG Xuqiong, YANG Jinyi, WANG Hong. Advances in Diagnosis Technologies of Viral Animal Epidemic Diseases[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 294-306.

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Advances in Diagnosis Technologies of Viral Animal Epidemic Diseases

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