一株鸡源性肠球菌的鉴定及耐药机制分析

Abstract

Abstract: This experiment was aimed at identifying a Broad-spectrum antibiotic resistant bacteria separated from chickens and discussing the mechanism of antibiotic resistance of the bacteria from the viewpoint of molecular biology. Identify the training characteristics and biochemical experiments of the bacteria based on the standard of Common Bacteria System Identification Manual. A pair of 16S rDNA general primer was designed to amplify 16S rDNA and sequenced. 12 pairs of primer amplify resistance gene was designed based on the results of sensitive method of antibiotic test. The results of training characteristics and biochemical experiments of the bacteria showed that they were Enterococcus. A target set of 1465 bp was amplified by PCR. Sensitivity tests showed that the bacteria tolerate several antibiotics. The results of PCR showed that there exist ant(6)-iv, OXA-10, tetM, CTX-M-1, TEM gene, so it was Enterococcus. The existence of resistance gene was one of the reasons why the bacteria generate resistance characteristics.

Key words: Enterococci; identification; drug-resistance; drug-resistance gene

CLC Number:

Q852.61⁺1

WANG Tao, DU Dong-dong, ZHUANG Yan-fei, CHANG Wei-shan. Isolation, Identification and Drug Resistance Analysis of a Pathogenic Enterococcus Strain Isolated from Chicken[J]. , 2012, 39(6): 165-168.

References

1 陈一资,蒋文灿,胡滨. 对鸭场爆发罕见的粪链球菌的研究[J]. 中国兽医学报, 2003, 23(4):324~325.
2 东秀珠,蔡妙英.常见细菌系统鉴定手册[M]. 北京:科学出版社,2001,244~258.
3 施金玲,彭少华,李从荣. 肠球菌生物学性状及耐药性的调查[J]. 中国人兽共患病杂志, 2002, 18(5):75~77.
4 吴信明,苗立中,李峰,等.商品肉鸭链球菌病的诊治[J]. 中国家禽, 2003, 25(20):14.
5 Coudron P M, Markowitz S M, Wong E S, et al. Isolate of beta-lactamase-producing, aminoglycoside-resistant strain of Enterococcus faecium[J].Antimicrob Agents Chemother,1992,36:1125~1126.
6 Linden P K,Miller C B. Vancomycin-resistant Enterococci: the clinical effect of a common nosocomial pathogen. Diagn Microbiol Infect Dis, 1999, 32 (2): 113~120.
7 Maschieto A, Martine R, Palazzo I C, et al. Antimivrobial resistance of Enterococcus sp.isolated. from the intestinal tract of patients from a university hospital in Brazil[J].Mem Inst Oswaldo Cruz,2004,99(7):763~767.
8 Moellering Jr Rc. Emergence of Entercoccus as a signficant pathogen [J]. Clin Infect Dis, 1992, 14(6): 1173.
9 Murray B E, Lopardo H A, Rubeguo E A, et al. Intrahospital spread of a single gentamicin-resistance, beta-laetamase-producing strain of Enterococcus faecalis in Argentina[J]. Antimicrob Agents Chemother, 1992, 36:230~232.
10 Murray P R. Manual of clinical microbiology[M]. 7th Washington D C:Am Soc Microbiol,1999,297~305.
11 Piekarska K, Lawrynowicz-Paciorek M, Magdziak A, et al. Drug sensitivity of Enterococcus sp.strains isolated from clinical material or from healthy persons[J]. Med Dosw Microbiol,2002,54(4):305~315.
12 Rice L B. Tn916 family conjugative transposons and dissemination of antimicrobial resistance determinants[J]. Antimicrob Agents Chemother, 1998, 42(8):1871~1877.
13 Rosenberg J, Tenover F C, Wong J, et al. Are clinicallaboratories in California accurately reporting vancomycin- resistant enterococci[J]. J Clin Microbiol,1997,35(10):2526~2530.

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Isolation, Identification and Drug Resistance Analysis of a Pathogenic Enterococcus Strain Isolated from Chicken

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