FGF22及其受体在庆阳黑山羊正常睾丸与隐睾中的表达比较

doi:10.16431/j.cnki.1671-7236.2021.04.022

Abstract

Abstract: This experiment was conducted to compare the distribution of the fibroblast growth factor 22 (FGF22),fibroblast growth factor receptor 2 (FGFR2),and the related regulatory factors heparan sulfate proteoglycans (HSPG) in normal testis and cryptorchidism of Qingyang Black goat,and explore their roles in testicular development and cryptorchidism.HE staining and special staining were used to observe the histological structure of FGF22,FGFR2 and HSPG,and the localization of FGF22,FGFR2 and HSPG in goat normal testis and cryptorchidism was studied by immunohistochemistry and immunofluorescence combined with morphometry.The results showed that the seminiferous tubules of cryptorchidism were narrower than those of normal testis,the arrangement of spermatogenic cells was disordered,the collagen fibers and reticular fibers in interstitium were increased,and the positive reaction of glycogen was weak.The overall expression density of FGF22 in Leydig,Sertoli cells,peritubular myoid cells and vascular endothelial cells of cryptorchidism was significantly lower than that of normal testis (P<0.05).The expression of HSPG in normal testis was significantly higher than that in cryptorchidism (P<0.05),especially in interstitial tissue.The expression of FGFR2 was significantly increased in cryptorchidism group (P<0.05),and Sertoli cells were the main positive cells.The results showed that the cryptorchidism of Qingyang Black goat was more abnormal than that of normal testis,the interstitial tissue had a tendency of fibrosis,and the content of glycogen decreased.The decreased expression of FGF22 and HSPG should be closely related to the changes of local environment temperature of cryptorchidism.The increased expression of FGFR2 in cryptorchidism group suggested that it might be regulated adaptively by Sertoli cells during cryptorchidism.

Key words: Qingyang Black goat; testicles; cryptorchidism; FGF22; FGFR2; HSPG

CLC Number:

S814.1

SONG Juanjuan, YUAN Ligang, WANG Qianmei, CHEN Shaoyu, GUO Yan. Comparison of the Expression of FGF22 and Its Receptor in Normal Testicles and Cryptorchidism of Qingyang Black Goat[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1351-1360.

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Comparison of the Expression of FGF22 and Its Receptor in Normal Testicles and Cryptorchidism of Qingyang Black Goat

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