成纤维细胞生长因子21及其受体1、2在小鼠毛囊形成期的定位和表达

doi:10.16431/j.cnki.1671-7236.2019.01.003

Abstract

Abstract:

This study had investigated the localization and expression of fibroblast growth factor 21 (FGF21) with its receptors FGFR1 and FGFR2 in the formation phase of embryonic mice hair follicles.E13-E18 embryonic mice back skin were used to determine the mRNA and protein relative expression levels of FGF21,FGFR1 and FGFR2 by immunohistochemistry,Real-time PCR and Western blotting analysis.The results showed that:①FGF21 expressed in epidermis,basal layer and connective tissue at E13;Expressed in epidermis and placode at E14;Slightly expressed in hair bud at E15;Expressed in hair peg at E16 and E17;Mainly expressed in immature hair follicle at E18.FGFR1 and FGFR2 expressed in epidermis,basal layer,placode,hair bud,hair peg,immature hair follicle and connective tissue at E13-E18.②The results of Real-time PCR and Western blotting showed that the relative expression levels of FGF21 mRNA and protein were higher at E14 than that of any other stages;The relative expression levels of FGFR1 mRNA and protein were higher at E16-E17;And the relative expression of FGFR2 mRNA and protein showed ascending trend from E13 to E18 and was highest at E18.In summary,during the embryonic hair follicle formation and development,FGF21 might play a role in inducing hair follicle activation;FGFR1 might promote hair peg formation;FGFR2 might be necessary to induce hair follicle formation and hair follicle maturation.

Key words: FGF21; FGFR1; FGFR2; embryonic mice; hair follicle formation stage

CLC Number:

Q954

LU Na, CAO Xiaorui, WU Jinqiang, YAN Ruiqin, ZHANG Jiaojiao, WU Jiahao, HE Xiaoyan. Localization and Expression of FGF21,FGFR1 and FGFR2 in Mice Hair Follicle Formation[J]. , 2019, 46(1): 20-27.

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Localization and Expression of FGF21,FGFR1 and FGFR2 in Mice Hair Follicle Formation

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