黔北麻羊UCP2基因组织表达及其多态性与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2020.07.014

Abstract

Abstract: This study was aimed to investigate the genetic polymorphism of uncoupling protein 2 (UCP2) gene and its correlation with growth traits in Qianbei Ma goats,in order to provide theoretical basis for breed improvement of Qianbei Ma goats.This experiment took 200 Qianbei Ma goats aged 6 months of age as the research object,SNP of UCP2 gene in Qianbei Ma goats was detected by direct sequencing,and the correlation between UCP2 gene and the growth traits of Qianbei Ma goats was analyzed.The expression of UCP2 gene in different tissues of Qianbei Ma goats was detected by Real-time quantitative PCR.The results showed that there were 4 SNPs in UCP2 gene,which were g.29614172 A>G,g.29619320 G>T,g.29619721 C>T and g.29620037 A>G,all of which produced 3 genotypes.Among them,g.29619320 G>T and g.29620037 A>G were missense mutations,g.29619320 G>T mutation caused glycine (Gly) to cysteine (Cys),and g.29620037 A>G mutation led to threonine (Thr) was mutated to alanine (Ala).The polymorphic information content showed that 4 SNPs were all moderate polymorphism (0.25 < PIC < 0.5).χ² test results showed that g.29614172 A>G,g.29619721 C>T and g.29620037 A>G mutation sites were in Hardy-Weinberg equilibrium in Qianbei Ma goats population (P>0.05),g.29619320 G>T mutation site extremely significantly deviated from Hardy-Weinberg equilibrium (P<0.01).The linkage disequilibrium analysis showed that g.29619320 G>T and g.29619721 C>T sites had strong linkage effects.Real-time quantitative PCR results showed that UCP2 gene was expressed to varying degrees in all tissues of Qianbei Ma goats.The highest expression level was in lung,followed by spleen,liver and heart,and the other tissues had lower expression level.The results of association analysis showed that the body weight,body oblique length,chest width,chest depth,bust and tube circumference of AG and GG genotypes at g.29614172 A>G were significantly higher than that of AA genotype,the chest depth,bust and tube circumference of TT genotype at g.29619320 G>T were significantly higher than that of GT and GG genotypes,the body height of CT genotype at 29619721 C>T was significantly higher than that of CC and TT genotypes,the body weight,chest depth and tube circumference of GG genotype at g.29620037 A>G were significantly higher than that of AG and AA genotypes (P<0.05).The results initially revealed that UCP2 gene had significant effects on some growth traits of Qianbei Ma goats,and 4 SNPs could be used as genetic markers for economic trait selection of Qianbei Ma goats.

Key words: Qianbei Ma goats; UCP2 gene; growth traits; tissue expression

CLC Number:

S813.3

AI Jinxin, LONG Anju, LUO Weixing, CAI Huifen. Tissue Expression of UCP2 Gene and Its Association Analysis Between Polymorphism and Growth Traits in Qianbei Ma Goats[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2093-2103.

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Tissue Expression of UCP2 Gene and Its Association Analysis Between Polymorphism and Growth Traits in Qianbei Ma Goats

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