体外法研究硝酸钠调控水牛瘤胃甲烷生成对脂肪酸生物氢化途径的影响

doi:10.16431/j.cnki.1671-7236.2020.07.012

Abstract

Abstract: The objective of this study was to investigate the effects of sodium nitrate on methane production and fatty acid hydrogenation process of buffalo was studied by in vitro batch fermentation.Three buffaloes weighted (650±50) kg with permanent rumen fistula were selected as the donors of rumen fluid.The ratio of concentrate to forage of substrate was 40:60.Four groups (5 replicates in each group) were set up,each group was added with 0.25 mg/mL α-Linolenic acid,and the level of sodium nitrate was 0,1,2 and 3 mg/mL respectively.The gas production and methane production were measured at 3,6,9,12 and 24 h respectively,and the fermentation parameters and fatty acid content were measured at the end of 24 h respectively.The results showed that:① Adding sodium nitrate significantly reduced the total gas production,CH₄ content and the ratio of methane/total gas production in rumen culture medium for 24 hours (P<0.05).Adding 1,2 and 3 mg/mL sodium nitrate reduced the methane content by 89.62%,91.20% and 91.75% respectively.② The pH and NH₃-N content of rumen culture medium added with sodium nitrate were significantly higher than that of the control group (P<0.05),the MCP content of 1 mg/mL sodium nitrate was also significantly higher than that of the control group (P<0.05),and the difference of other groups was not significant (P>0.05);The difference of acetic acid concentration added with sodium nitrate was not significant (P>0.05),but the concentrations of propionic acid,butyric acid,isobutyric acid,valeric acid and isovaleric acid were significantly lower than that of the control group (P<0.05),the acetic acid/propionic acid was significantly higher than that of the control group (P<0.05),and the total volatile fatty acid added with 3 mg/mL sodium nitrate was significantly lower than that of the control group (P<0.05).③ The content of C18:2 cis-9,trans-11,C18:2 trans-10,cis-12,C20:1,UFA and UFA/SFA in the group with 1 mg/mL sodium nitrate was significantly higher than that in the other groups (P<0.05);the content of C18:2n6c,C18:1n9t,C20:5n3 (EPA) and C22:6n3 (DHA) in the group with 1 mg/mL sodium nitrate was the highest,but the difference was not significant (P>0.05).The contents of C18:3n3,C18:2n6c and C18:1n9c in sodium nitrate groups were higher than those in the control group (P>0.05).It could be seen that the addition of sodium nitrate in vitro significantly reduced the total gas production and methane content,increased the pH and NH₃-N content,decreased TVFA content,increased acetic acid/propionic acid by significantly reducing propionic acid content.The addition of 1 mg/mL sodium nitrate significantly increased the content of CLA and UFA in rumen fluid,and inhibited methane,it could reduce the hydrogenation degree of unsaturated fatty acids at the same time.

Key words: sodium nitrate; methane; fatty acid hydrogenation; in vitro fermentation

CLC Number:

S823.8⁺3

GUO Yanxia, LI Mengwei, PENG Lijuan, PENG Kaiping, TANG Zhenhua, LIANG Xin, XIE Fang, YANG Chengjian. Effects of Sodium Nitrate on Methane Production and Fatty Acid Hydrogenation Process of Buffalo in vitro Fermentation[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2071-2080.

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Effects of Sodium Nitrate on Methane Production and Fatty Acid Hydrogenation Process of Buffalo in vitro Fermentation

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