新城疫病毒胶体金免疫层析试纸条的制备

›› 2009, Vol. 36 ›› Issue (9): 122-125.

• 疾病防治 • Previous Articles Next Articles

The Research of Gold Immunochromatographic Strip for Newcastle Disease Virus

LI Bei-bei^1,2, ZHANG Fan¹, XUE Qiang¹, LI Jin-feng¹, ZOU Ming-qiang¹, YANG Yi²

（1.Chinese Academy of Inspection and Quarantine, Beijing 100123, China; 2.Department of Chemistry, Beijing University of Chemistry and Technology, Beijing 100029, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
Contact: ZOU Ming-qiang

Abstract

Abstract: To develop a simple, rapid and high efficiency immunochromatographic assay for Newcastle disease virus (NDV). The sodium citrate reduction was used to make colloidal gold particles which will conjugate with NDV monoclonal antibody as immunoassay probes. The test line was sprayed on the nitrocellulose membrane, while a sheep anti-mouse antibody quality control line (C line) was sprayed. The strip could detect NDV in 10 min. The detection of NDV was more sensitive than the HA test results by improving 8 times. NDV had no cross-reactions with AIV. With buffer as control testing, the result was negative. Storage of the strips in a sealed, dry, low temperature conditions had not changed their sensitivity and specificity. The research revealed that the strip for detection of NDV has high specificity, sensitivity and stability. Its rapid detection and easy operation make it suitable for widely use. 

Key words: Newcastle disease virus; immunochromatographic strip; colloidal gold

CLC Number:

S851

LI Bei-bei;ZHANG Fan;XUE Qiang;LI Jin-feng;ZOU Ming-qiang;YANG Yi. The Research of Gold Immunochromatographic Strip for Newcastle Disease Virus[J]. , 2009, 36(9): 122-125.

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