Abstract: Splenic lymphocyte isolated and cultured from cattle spleen was exposed to different of levels of sodium fluoride in 24 h. Detecting GSH-Px activity, SOD activity, NOS avtivity and contents of MDA and NO of splenic lymphocyte. The results showed that GSH-Px activity and SOD activity were higher and other indexes were lower in the lymphocyte exposed to fluorine than control ones and there was doseeffect relationship. Conclusion: it is demonstrated that fluorine could degrade antioxdative activity, aggravate the level of lipid peroxidation, increase the content of free radicals, and induce the damage of lymphocyte.

Key words: fluorine; cattle; splenic lymphocyte; antioxdative activity