Abstract: Mammary gland epithelial cells have special functions of synthesis and lactation. These functions make them mammary bioreactor harrow cell. Experimental model made from mammary gland epithelial cells could actually response many biology mechanisms, such as mammoplasia,development, lactotroph, and validate if the construction of tissue specificity expression vector is rational and effective. It relates to cell biology, developmental biology,endocrinology, biochemistry and molecular biology and other fields. It is significant to the gland bioreractor, and the research of the lactoprotein gene expression. This article analyze the culture method of mammary epithelial cells.

Key words: mammary epithelial cells; in vitro; culture method 