Abstract: To develop a quick, simple and specific method for the detection of MHV which cause hepatitis of mice,colloidal gold was prepared by reduction of HAuCl₄ with trisodium citrate and label with purified MHV-Ab against MHV. The qualification and conditions of GICAs were selected and optimized. So the GICA method was built to detect MHV. Colloidal gold with about 20 nm particle diameter, the least amount of antibody to stabilize the 0.5 mL colloidal gold is 5.05 μg. We chose M135 as the chromatography material, and 3% BSA as the confining liquid. The most appropriate amount of test line antibody protein is 640 μg/mL, and the amount of quality control line antibody protein is 800 μg/mL. The method could detect the MHV about 10 minutes with specificity. While positive result, the test line and the quality control line were red. As for negative result, only the quality control line was red. The method of GICA in this paper could detect the MHV rapidly with specificity and stability.

Key words: MHV; MHV-Ab; colloidal gold; GICA