白绒山羊PH-30基因的克隆及表达

›› 2010, Vol. 37 ›› Issue (9): 135-137.

• 遗传繁育 • Previous Articles Next Articles

Cloning and Expression of Cashmere Goat PH-30 Gene

FU Lin，NA Ren-hua

(College of Animal Science，Inner Mongolia Agricultural University，Hohhot 010018，China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
Contact: NA Ren-hua

Abstract

Abstract: PH-30 is the best characterized molecule involved in the sperm-egg plasma membrane interaction. In this study, total RNA was prepared from cashmere goat testis and different parts of epididymis, cDNA fragment was amplified by RT-PCR using specific primers,then PCR product was cloned into pGM-T Easy vector. The nucleotide was sequenced and analyzed, 99% identity with sheep PH-30 and 92% homology with bovine PH-30 were found. Expression analysis by RT-PCR revealed that PH-30 was only expressed in the testis when compared to caput, corpus, cauda epididymis.

Key words: PH-30 gene; RT-PCR; cloning; mRNA expression

CLC Number:

Q78

FU Lin;NA Ren-hua. Cloning and Expression of Cashmere Goat PH-30 Gene[J]. , 2010, 37(9): 135-137.

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Cloning and Expression of Cashmere Goat PH-30 Gene

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