China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (11): 108-113.

• 遗传繁育 • Previous Articles     Next Articles

Study on the Developmental Competence of Equine Oocytes after ICSI and the Corpus Luteum Ultrastructure during Post-pregnancy Period in Mare

WANG Rong-xiang1,2， YE Xiao-fang2,3， ZHANG Guo-ting2，CHEN Jing-bo2   

  1. (1.The College of Animal Sciences， Xinjiang Agricultural University， Urumqi 830052， China； 2.Institute of Animal Sciences， Xinjiang Academy of Animal Sciences， Urumqi 830000， China；3.College of Animal Science and Technology, Shihezi University， Shihezi 832003， China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-11-20 Published:2009-11-20
  • Contact: CHEN Jing-bo

Abstract: This study was conducted to evaluate the effect of initial cumulus morphology (expanded or compact)，systems of in vitro maturation (TCM 199 or NCSU 23) and duration of in vitro maturation (34 or 38 h) on the developmental competence of equine oocytes after intracytoplasmic sperm injection (ICSI) in non-breeding season.Oocytes were collected from slaughterhouse-derived ovaries，matured in vitro，and injected with frozen-thawed stallion sperm. The study on the developmental competence of equine oocytes after ICSI indicated that：① The maturation rates of expanded (Ex) oocytes were significantly higher than those of compact (Cp) oocytes (P<0.05，61.09% vs 41.24%).Thirty-six hours after ICSI of mature oocytes，the rate of cleavage with normal morphology was no significant difference with expanded or compact oocytes (P>0.05，47.34% vs 44.92%). ② Two different systems of in vitro maturation did not affect development of embryos from expanded or compact oocytes at 36 h after ICSI (P>0.05).The maturation rates of expanded oocytes were significantly higher than compact oocytes when matured in the same system (P<0.05)，but there is no significant difference for the rate of cleavage with normal morphology at 36 h after ICSI(P>0.05). ③ The maturation rates were higher for oocytes matured for 38 h than for those matured for 34 h (44.43% to 68.87% vs 34.52% to 58.90%). The rate of cleavage with normal morphology at 36 h after ICSI with or without activation were significantly higher for expanded oocytes matured in TCM 199 for 34 h than matured for 38 h with activation (P>0.05)，for compact oocytes matured in TCM 199 for 34 h with activation(P<0.05)，and for expanded oocytes matured in NCSU 23 for 38 h without activation (P<0.05). ④ Yet，combined activation with ionomysin and 6-DMAP did not affect the rate of cleavage with normal morphology at 36 h after ICSI (P>0.05).These data indicate that developmental competence differs between expanded and compact equine oocytes，and is differentially affected by the duration of maturation.

Key words: mare; ICSI; assisted reproductive technology; in vitro fertilization

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