不同鸡马立克病疫苗的免疫保护效果比较

doi:10.16431/j.cnki.1671-7236.2024.03.039

Abstract

Abstract: 【Objective】 To understand the immune protection effects of different commercial Marek’s disease vaccines,and provide valuable data for Marek’s disease prevention and control,this study analyzed the immune protection of 7 batches/varieties (A-D,F-H) of CV1988 or CVI988＋HVT commercial vaccine.【Method】 A total of 140 1-day-old SPF chickens were randomly divided into 9 groups,groups G01 to G07.G01 to G07 (15 chickens/group) were immunized with Marek’s disease vaccine A-D and F-H according to the manufacturer’s recommended dose,respectively,while groups G08 (challenge control group,15 chickens) and G09 (blank control group,20 chickens) were not immunized.At 7 days of age,the chickens of groups G01 to G08 were intraperitoneally injected with Md5 strain (1 000 PFU/bird).All chickens were raised to 120 days of age.The protective effect of each vaccine was evaluated through the examination of virus plaque numbers,vaccine replication,weight differences post-challenge,clinical manifestations,and pathological changes.【Result】 The virus plaque number of all 7 vaccine batches confirmed to national standard (2 000 PFU/feather),and the virus plaque number of vaccine F (6 467 PFU/feather) was the highest.The replication rate of vaccine F was faster than other vaccines.After challenged with Md5 strain,the weight of the challenge control group chickens were decreased,and the unvaccinated chickens challenged with Md5 had 93.3% (14/15) mortality,and 100% (15/15) MD lesions.Compared with blank control group,there was no significant difference in body weight in groups G01 to G07 (P>0.05). The mortality of vaccinated chickens in groups G01-G07 were 0/14,3/15,5/15,0/14,0/13,0/15 and 1/15,respectively.The MD lesion proportion of vaccinated chickens in groups G01-G07 were 5/14,8/15,7/15,5/14,3/13,3/15 and 5/15,respectively.The results of immune challenge protection test showed that 7 batches of Marek’s disease vaccine had a certain protective effect on chickens,and the protection index of these vaccines were 45.7%-73.3%.【Conclusion】 Different manufacturer’s Marek’s disease vaccine demonstrated comparable protective effect against Md5 strain of MDV.Furthermore,the bivalent vaccine exhibited higher protection (73.3%) compared to the monovalent vaccine.

Key words: Marek’s disease; commercial vaccine; challenge protection test; protection index

CLC Number:

S859.79⁺7

YANG Huiming, PU Xing, DANG Haibin, SUN Lu, ZHANG Guozhong. Comparative Evaluation of Immunological Protective Efficacy Among Various Marek’s Disease Vaccines in Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1267-1276.

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Comparative Evaluation of Immunological Protective Efficacy Among Various Marek’s Disease Vaccines in Chickens

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