干扰素对鸡胚内H9N2亚型禽流感病毒滴度的影响

Abstract

Abstract: To investigate effect of interferon on proliferation of H9N2 avian influenza virus in chick embryo, chick embryos were grouped and treated with interferon and H9N2 avian influenza virus (AIV). And then virus titer was detected by using HA. The results showed that proliferation of AIV could be restrained in the high-dose group, middle-dose group and low-dose group, but there was no significant difference among the three treated groups. The high-dose group interferon could significantly inhibit the proliferation of the virus, which compared with other dose groups in a certain time. The results showed that the high-dose interferon inhibited the proliferation of H9N2 AIV obviously in a certain period of time, this would have a certain application value in the production.

Key words: interferon; chick embryo; H9N2 avian influenza virus

CLC Number:

S859.79⁺7

WANG Xin-wei;WANG Yu;LI Jian-zhong;WANG Yan. Effect of Interferon on Proliferation of H9N2 Avian Influenza Virus in Chick Embryo[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(3): 77-79.

[1]	JIANG Xinrui, FENG Helong, YANG Hongchun, JIANG Liren, WANG Hongcai, ZENG Zhe, ZUO Bo, ZHANG Tengfei, LUO Qingping, SHANG Yu, WEN Guoyuan. Effect of Bamboo Leaf Flavonoid on Immune Efficacy of Chick Embryo Inoculation with Newcastle Disease Vaccine [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 4174-4181.
[2]	WAN Lijun, WANG Sheng, XIE Zhixun, REN Hongyu, XIE Liji, FAN Qing, LUO Sisi, LI Meng, ZHANG Yanfang, ZENG Tingting, HUANG Jiaoling, ZHANG Minxiu, XIE Zhiqin, LI Xiaofeng, WEI You. Dynamic Changes in Expression of Interferon-stimulated Genes at the Transcriptional Level After Infection with Fowl Adenovirus Serotype-4 in LMH cells [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 2081-2090.
[3]	IN Huan, MENG Zhaoying, TU Min, SHEN Jia, SHI Aihua, ZHAO Lei, XI Shuo, ZHANGJianwei, ZHANG Zhenhua. Study on the Interaction Between Chicken Interferon γ and Complement Receptor 2 Protein [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4476-4484.
[4]	DENG Zhichao, ZHANG Cheng, LUO Pei, ZHAN Xiaozhi, JIANG Danli, YANG Youtian, LIU Wenjun. Cloning and Tissue Expression Specificity Analysis of Interferon Regulatory Factor 7 Gene in Ducks [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(7): 2606-2616.
[5]	SONG Shiying, GUO Weilu, XIA Xuefeng, ZHANG Xue, BI Zhenwei, ZHANG Xuehan, FAN Baochao, DONG Hailong, LI Bin. Expression and Purification of Porcine IFN-δ5 and Its Effect Analysis Against PEDV Infection [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(8): 3171-3179.
[6]	YU Jie, HU Xiuzhong, XIANG Min, WANG Dingfa, TAO Bifei, XIA Yu, TAO Hu, XIONG Qi, CHENG Lei. Effects of Arginine on Expression of Genes in Bovine Endometrial Epithelial Cells Treated with Interferon-tau [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(7): 2495-2503.
[7]	YAN Kexin, DENG Zhibang, HE Jiayi, YUAN Qichao, WANG Jiaoshun, YUAN Xiaomin. Effect of Interferon-inducible Transmembrane Protein 1 on the Adsorption of Porcine Coronavirus to Host Cells [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(4): 1370-1380.
[8]	WANG Sheng, WAN Lijun, XIE Zhixun, XIE Liji, LUO Sisi, FAN Qing, ZHANG Yanfang, ZENG Tingting, HUANG Jiaoling, ZHANG Mingxiu, XIE Zhiqin, DENG Xianwen. Altered Gene Expression Profiles of the Interferon Stimulated Genes in DF1 Cells Infected with Avian Reovirus [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(4): 1423-1430.
[9]	ZHAI Junqiong, WU Yajiang, DAI Junwei, CHEN Anni, ZHOU Niu, WANG Chen, DU Xueqing, LUO Manlin, CHEN Wu. Cloning and Bioinformatics Analysis of the Siberian Tiger γ-interferon Gene and Its Expression in Pichia pastoris [J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(1): 72-82.
[10]	LI Jiajia, LI Suhua, SONG Jianling, HUANG Jingjun, MIAO Yuan, CHEN Xi, JI Jia. Sequence Analysis of HA and NA Genes of Two H9N2 Subtype Avian Influenza Virus in Yunnan [J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(3): 873-883.
[11]	HUANG Huilan, LI Wenjun, XIE Zimin, YANG Huihu, CUI Huiyi, HUANG Shujian, ZHANG Xuelian. Molecular Cloning and Expression of Duck IFIT5 Gene and Preparation of Its Polyclonal Antibodies [J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(11): 3713-3721.
[12]	WANG Jing, YANG Haifeng, WANG Dada, CHEN Xiaolan. The Application and Research Progress of Chicken Embryo and Avian Cell Lines in Biomedicine [J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(11): 3783-3791.
[13]	ZHU Hongxiao, WANG Qiuxia, LI Peng, OU Changbo, HU Huilong, WEI Xiaobing, ZHANG Xiulin, LIU Xingyou. Research Progress on Mechanism of Porcine Epidemic Diarrhea Virus Evading the Antiviral Effect of Host Interferon [J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(7): 2096-2102.
[14]	SONG Tianqi, HOU Shaohua, GUO Xiaoyu, XIN Ting, JIANG Yitong, YUAN Weifeng, ZHU Hongfei, JIA Hong. Yeast Expression of Recombinant Canine Alpha Interferon 6 and Evaluation of Their Antiviral Activities [J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(7): 2144-2150.
[15]	ZHANG Shuang, FAN Shuangshuang, CHANG Wenru, DING Guangxu, GUO Ruizhen, DUAN Lifang, DU Yongkun, CHU Beibei, YANG Guoyu, WANG Jiang. Effect of Interferon Regulatory Factor 3 Gene Knockout on Proliferation of Pseudorabies Virus [J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(5): 1253-1262.

Effect of Interferon on Proliferation of H9N2 Avian Influenza Virus in Chick Embryo

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments