牛TGFB1基因克隆、生物信息学及组织表达分析

doi:10.16431/j.cnki.1671-7236.2025.06.005

Abstract

Abstract: 【Objective】 The aim of this experiment was to clone the CDS region of bovine transforming growth factor beta 1 (TGFB1) gene and explore its expression in different tissues and adipocytes during differentiation of Guyuan cattle,laying a foundation for the subsequent research on the function of TGFB1 gene.【Method】 The CDS region of bovine TGFB1 gene was amplified by PCR using adipose tissue cDNA as template.The physicochemical properties,subcellular localization,protein structure and functional domain of TGFB1 protein were predicted by bioinformatics online softwares.Real-time quantitative PCR was used to detect the expression of TGFB1 gene in bovine adipocyte during differentiation and different tissues.【Result】 The CDS region of bovine TGFB1 gene was successfully cloned with a total length of 1 173 bp.The protein encoded by TGFB1 gene was mainly composed of 390 amino acids and its chemical formula was C₁₉₈₁H₃₁₃₄N₅₅₄O₅₆₄S₂₁.The total average hydrophilic (GRAVY) value was ―0.328.TGFB1 protein had 37 phosphorylation sites,49 O-glycosylation sites and 3 N-glycosylation sites.The secondary structure of TGFB1 protein was mainly composed of alpha helix and random coil,and it interacted with SMAD2,SMAD3,ITGAV and other proteins.Real-time quantitative PCR results showed that compared with 0 d after induction differentiation，TGFB1 gene expression was significantly decreased at 2 d (P＜0.05),and significantly increased at 4-10 d (P＜0.05).Tissue expression profile showed that the expression of TGFB1 gene was the highest in spleen of calves and subcutaneous fat of adult cows,respectively,and was significantly higher than that in other tissues (P＜0.05). 【Conclusion】 The expression level of the bovine TGFB1 gene was relatively high at 4 d of bovine precursor adipocytes, and it was highly expressed in spleen of calves and subcutaneous fat of adult calves. It played an important role in regulating the proliferation and differentiation of adipocytes and the physiological processes of the calf immune system. This results provided a theoretical basis for further exploring the molecular mechanism of the TGFB1 gene involved in fat deposition in Guyuan cattle.

Key words: Guyuan cattle; TGFB1 gene; bovine preadipocytes; tissue expression

CLC Number:

S823

CHU Hongen, LIU Yuan, FENG Xue, BAI Xue, YANG Mengli, LI Juan, HE Lixia, LIU Shuang, FENG Lan, MA Yun. Cloning,Bioinformatics and Tissue Expression Analysis of Bovine TGFB1 Gene[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2506-2518.

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Cloning,Bioinformatics and Tissue Expression Analysis of Bovine TGFB1 Gene

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