丽江猪HMOX2基因扩增、序列分析及组织表达研究

doi:10.16431/j.cnki.1671-7236.2025.05.026

Abstract

Abstract: 【Objective】 This study was aimed to amplify and explore the coding sequence (CDS) sequence characteristics of heme oxygenase 2 (HMOX2) gene in Lijiang pigs, and analyze its expression in different growth stages and tissues of Lijiang pigs,establishing a foundation for molecular marker-assisted breeding utilizing HMOX2 gene.【Method】 The HMOX2 gene sequence of Lijiang pigs was amplified by PCR.The physicochemical properties,codon preferences,and predicted protein structure were analyzed using bioinformatics software.The expression of HMOX2 gene were detected using Real-time quantitative PCR in heart,liver,spleen,lung,kidneys,and longissimus dorsi muscle of 2-month-old Lijiang pigs,as well as in the subcutaneous fat of 2-,4-,and 6-month old Lijiang pigs,and compare the expression of HMOX2 gene in subcutaneous fat of 2-month-old Lijiang pigs and Duroc pigs.【Result】 The CDS of HMOX2 gene in Lijiang pigs was found to be 951 bp long,encoding 316 amino acids,and the codon prefers to end with C or G.The HMOX2 gene in Lijiang pigs showed high similarity to Bos taurus, Bos indcus, Capra hircus and Ovis aries (90.1%-91.2%),and the lowest similarity to Gallus gallus (71.3%).Among six local pig breeds,except Bamei pigs and Rongchang pigs, HMOX2 gene in Lijiang pigs showed 100% similarity to local pigs,and the similarity with five exotic pigs was 99.8%.Two base synonymous mutations were present in the CDS region of HMOX2 gene in Lijiang pigs compared with Duroc pigs. HMOX2 protein were determined to be stable transmembrane hydrophilic proteins without a signal peptide,featuring one transmembrane structure,primarily functioning in cytoplasm,and containing 21 phosphorylation and 33 glycosylation sites.The secondary structure of HMOX2 protein was predominantly composed of alpha helix (54.43%) and random coil (42.00%).Protein interaction network analysis demonstrated that HMOX2 protein might interact with 10 proteins,including FECH and HMOX1,primarily playing a role in lipid metabolism.Real-time quantification PCR results indicated that HMOX2 gene was expressed in heart,liver,spleen,lung,kidney,longissimus dorsi muscle,and subcutaneous fat of Lijiang pigs,and the expression increased with the increase of age in subcutaneous fat.Compared with Duroc pigs,the expression of HMOX2 gene in subcutaneous fat of Lijiang pigs was significantly higher than that of Duroc pigs (P＜0.05).【Conclusion】 HMOX2 gene in Lijiang pigs was successfully amplified,and its molecular characteristics were elucidated.It was expressed in seven tissues such as lung,liver,and subcutaneous fat,and its expression in subcutaneous fat increased significantly with the increase in monthly age.These results provided a foundation for further investigation into the role of HMOX2 gene in fat deposition in Lijiang pigs.

Key words: Lijiang pigs; HMOX2 gene; sequence analysis; tissue expression

CLC Number:

S828

LI Tianxiu, LI Xinpeng, DONG Xinxing, LAN Guoxiang, YAN Dawei, ZHU Jiawei. Amplification,Sequence Analysis and Tissue Expression Study of HMOX2 Gene in Lijiang Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(5): 2219-2231.

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Amplification,Sequence Analysis and Tissue Expression Study of HMOX2 Gene in Lijiang Pigs

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