China Animal Husbandry and Veterinary Medicine ›› 2025, Vol. 52 ›› Issue (4): 1455-1467.doi: 10.16431/j.cnki.1671-7236.2025.04.001

• Biotechnology • Previous Articles     Next Articles

Genome Wide Association Study of Immune Traits in Duroc×Erhualian F2 Generation Pigs

MIAO Na1, QIAO Jiakun1, YANG Hui1, HAN Pingping1, XU Fangjun1, CHE Zhaoxuan1, DAI Xiangyu1, XU Minghang1, LONG Zhiwei1, ZHU Mengjin1,2   

  1. 1. Key Labortory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China;
    2. The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2024-08-12 Published:2025-03-29

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Abstract: 【Objective】 Pigs play a crucial role in agriculture,and the analysis of their immune traits holds significant importance for enhancing production efficiency and disease resistance.The objective of this study was to identify potential candidate genes associated with immune traits in Duroc×Erhualian F2 generation hybrid pigs,thereby providing valuable gene information for breeding programs aimed at improving porcine disease resistance.【Method】 A total of 294 Duroc×Erhualian F2 generation hybrid pigs were collected from ear and tail tissue samples within 24 hours after birth,and genomic DNA was extracted.Blood samples were collected at 35 days of age to detect cytokines.Genome-wide association study (GWAS) was performed on the immune traits related to cytokines (IFN-α,IFN-γ,IGG,IL8 and IL10) in Duroc×Erhualian F2 generation hybrid pig resource population to identify single nucleotide polymorphism (SNP) site related to the traits.On this basis,the IFN-γ/IL10 value (IFNGIL10) was utilized for further identification of the related SNP and candidate gene influencing the immune traits of pigs.Subsequently,GO function and KEGG pathway enrichment analysis were performed on the identified related genes,and protein-protein interaction (PPI) analysis was performed on the proteins encoded by the candidate genes.【Result】 A total of 10 SNPs were identified through GWAS analysis,which specifically targeted 21 candidate genes associated with immune traits (CH25H,LIPA,SIGMAR1,PIP5KIC,PLCZ1,PIK3C2G,CNTFR,IL11RA,SAP30,HMGB2,CCL21,CCL19,CCL27,GNA11,GNA15,DSCC1,TERF2IP,FZR1,ENPP2,CACTIN and FAS).GO function enrichment analysis results indicated that these candidate genes were significantly enriched in terms such as chemokine activity,cellular response to interleukin-1 and inflammatory response.KEGG enrichment analysis results demonstrated that the candidate genes were significantly enriched in pathways such as cytokine-cytokine receptor interaction,inositol phosphate metabolism and calcium signaling pathway.Furthermore,the PPI analysis constructed a total of 12 subnetworks,suggesting potential interactions among these candidate genes,which were notably enriched within the same pathway,collaboratively participating in biological processes to fulfill their respective functions.【Conclusion】 The present study identified 10 SNPs associated with porcine immune traits through GWAS,and further selected 21 candidate genes for target traits by integrating enrichment analysis and PPI analysis.Overall,the findings from this study not only deepened our understanding of the molecular basis underlying porcine immune traits,but also provided a theoretical foundation for porcine disease resistance breeding.

Key words: pigs; cytokines; immune traits; genome-wide association study; candidate genes

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