猪源霍氏肠杆菌的分离鉴定及其耐药性、致病性分析

doi:10.16431/j.cnki.1671-7236.2025.01.034

Abstract

Abstract: 【Objective】 The purpose of this experiment was to investigate the resistance characteristics and pathogenicity of Enterobacter hormaechei in a large-scale pig farm in Yili,Xinjiang,and provide reference for the prevention and control of Enterobacter hormaechei. 【Method】 Bacteria was isolated and purified from the samples of anal and nasal swabs of pigs.The isolate was identified by Gram staining,morphological observation,colony morphological characteristics,biochemical characteristics,species-specific detection and 16S rRNA sequencing analysis.The sensitivity of the isolate to 20 commonly used antibiotics was determined by K-B method.The drug resistance genes and virulence genes were detected by PCR,and the pathogenicity was analyzed by animal test.The biofilm forming ability of the isolate was measured by glass tube method and microtitration plate method. 【Result】 A strain of bacteria was isolated and named H1.The isolate could form milky white colonies with protrusion,smooth and wet surface on the nutrient agar medium.Gram staining showed that the bacteria were red in short rod shape and scattered in arrangement.The results of biochemical identification showed that glucose,lactose,urea and other biochemical tests were positive,and hydrogen sulfide and phenylalanine were negative.PCR results showed that the target bands of Eh specific gene and 16S rRNA were 487 and 1 476 bp,respectively.Sequencing results showed that the similarity between the isolated strain and Enterobacter hormaechei reached 100%,and the isolated H1 was comprehensively determined to be Enterobacter hormaechei.The isolate was resistant to 15 antibiotics,such as enrofloxacin,flufenicol and cotrimoxazole,and sensitive to 5 antibiotics,such as polycolistin B and ciprofloxacin.It carried 8 drug resistance genes (bla_CTX-M-1,tem,sul1,sul2,sul3,tetA,tetM and qnrB genes) and 5 virulence genes (fimA,csdg,papD,ompA and cpa genes).The results of animal pathogenicity test showed that the isolte was pathogenic to mice,causing duodenum,liver,spleen and kidney damage after infection,and had strong biofilm formation ability. 【Conclusion】 In this study,a strain of Enterobacter hormaechei was isolated and obtained,which showed multiple drug resistance,carried multiple drug resistance genes and virulence genes,had certain pathogenicity to mice,and had strong biofilm formation ability.The results provided a scientific basis for the prevention and control of Enterobacter hormaechei.

Key words: pigs; Enterobacter hormaechei; drug resistance; pathogenicity

CLC Number:

S852.65

LI Nana, YU Xingyu, HOU Gongmingzhu, LI Yang, GUO Yaqi, ZHENG Pei, LI Yanfang, LIANG Yan, HE Gaoming, QU Yonggang. Isolation and Identification,Drug Resistance and Pathogenicity Analysis of Enterobacter hormaechei from Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 376-388.

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Isolation and Identification,Drug Resistance and Pathogenicity Analysis of Enterobacter hormaechei from Pigs

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