基于16S rDNA测序分析毛滴虫对猕猴肠道菌群结构的影响

doi:10.16431/j.cnki.1671-7236.2024.11.032

Abstract

Abstract: 【Objective】 This experiment was aimed to study the changes and diversity of gut microbiota in rhesus monkeys(Macaca mulatta) infected with Trichomonas using 16S rDNA sequencing technology,provide a reference for clinical research on using microbiota as an alternative antibiotics for treating trichomoniasis. 【Method】 Six samples of rhesus monkeys infected with Trichomonas and six samples of Trichomonas-negative fresh feces from rhesus monkeys were collected for predictive analysis of gut microbiota and functional genes through metagenomic sequencing. 【Result】 The diversity of gut microbiota in Trichomonas-infected rhesus monkeys decreased significantly compared to healthy rhesus monkeys,leading to significant changes in microbial community composition.Specifically,there was a significant decrease in Bacteroidota and Fibrobacterota (P＜0.05),while Campylobacta and Desulfobacterota showed a significant increase (P＜0.05).Additionally,Lactobacillales,Campylobacter and Campylobacteraceae were significantly enriched in the intestinal microbes of Trichomonas-infected rhesus monkeys,whereas Prevotella_9,Bacteroidales and Bacteroidota were significantly enriched in the intestinal microbes of Trichomonas-negative rhesus monkeys.Furthermore,Brachyspira_hampsonii and Prevotellaceae_bacterium_Marseille_P2831 were identified as biomarkers of Trichomonas-infected rhesus monkeys.The functional gene prediction of gut microbiota also revealed a significant decrease in the biosynthesis function of amino acids and antibiotics in the Trichomonas-infected rhesus monkeys (P＜0.05). 【Conclusion】 Infection with Trichomonas could lead to a reduction in the diversity of gut microbiota and an alteration in the composition of the microbial community in rhesus monkeys.Additionally,it could also decrease the biosynthetic function of gut microbiota.

Key words: Macaca mulatta; Trichomonas; 16S rDNA; gut microbiota; functional gene prediction

CLC Number:

S852.72

LI Heling, CHEN Zhigang, ZONG Faliang, NING Qing, YAN Yulin, WANG Hong. Effect of Trichomonas on the Structure of Macaca mulatta Gut Microbiota Based on 16S rDNA Sequencing Technology[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(11): 4974-4983.

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Effect of Trichomonas on the Structure of Macaca mulatta Gut Microbiota Based on 16S rDNA Sequencing Technology

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