基于cELISA检测的绵羊抗布鲁氏菌病性状全基因组关联分析

doi:10.16431/j.cnki.1671-7236.2024.10.001

Abstract

Abstract: 【Objective】 The aim of this study was to explore genes associated with brucellosis resistance or susceptibility in sheep，in order to provide molecular markers for brucellosis resistance breeding in sheep. 【Method】 In this study，sheep naturally infected with brucellosis and not vaccinated were used as research subjects，and blood samples were collected for competitive enzyme-linked immunosorbent assay (cELISA). The whole-genome resequencing was conducted on the blood DNA samples. The results of cELISA were used as quantitative trait phenotypes，and GEMMA software was used for genome-wide association study. The clusterProfiler package was used to perform enrichment analysis on significant genes. 【Result】 A total of 56 genes were annotated from the Top 1 000 loci selected by genome-wide association study. GO functional enrichment results showed that annotated genes were mainly concentrated in β-selection and αβ T cell proliferation and differentiation. KEGG pathway enrichment analysis showed that annotated genes were mainly concentrated in Th1/Th2/Th17 cell differentiation and natural killer cell-mediated cytotoxicity. It mainly involved 6 genes，including interferon gamma receptor 1 (IFNGR1)，nuclear factor of activated T cells 2 (NFATC2)，NF-κB inhibitor beta (NFKBIB)，spleen associated tyrosine kinase (SYK)，transforming growth factor beta receptor 2 (TGFBR2)，and Zeta chain of T cell receptor associated protein kinase 70 (ZAP70). The screened items，pathways，and genes were closely related to Brucella infection or the host’s anti-infection response. 【Conclusion】 6 genes related to sheep brucellosis were screened，which laid a foundation for further functional verification at the cellular or individual level，and provided a reference for molecular markers for breeding sheep brucellosis resistance.

Key words: sheep; brucellosis; competitive enzyme-linked immunosorbent assay (cELISA); genome-wide association study (GWAS)

CLC Number:

S813.2

WU Shangjie, LI Jiajia, JIANG Lin, MA Yuehui, DING Jiabo, HE Xiaohong. Genome-wide Association Study of Anti-brucellosis Traits Based on cELISA Detection in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4191-4199.

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Genome-wide Association Study of Anti-brucellosis Traits Based on cELISA Detection in Sheep

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