乌珠穆沁羊DGAT1、NR6A1、MAN1A1基因多态性与其生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.09.022

Abstract

Abstract: The aim of this study was to elucidate the polymorphisms of g. 13504098 C>T of diacylglycerol acyltransferase 1 (DGAT1) gene, g. 11204707 A>C of nuclear receptor subfamily 6 group A member 1 (NR6A1) gene and g. 18795097 C>T of alpha-mannosidase (MAN1A1) gene, and its association with the growth traits in Ujumqin sheep, providing new genetic markers for high productivity molecular breeding of Ujumqin sheep. g. 13504098 C>T, g. 11204707 A>C and g. 18795097 C>T of DGAT1, NR6A1 and MAN1A1 genes in Ujumqin sheep were detected by Sequenom MassARRAY^®SNP assay, and the linear mixed model was used to analyze the association between genotypes and the growth trait in Ujumqin sheep at 4 and 6 months of age. The results showed that three genotypes were detected at three loci. Specifically, g. 13504098 C>T included CC, CT and TT genotypes, CC genotype was dominant genotype (0.49), C was the dominant allele (0.70). g. 11204707 A>C included AA, CA and CC genotypes, AA genotype was dominant genotype (0.54), A was the dominant allele (0.73). g. 18795097 C>T included CC, TC and TT genotypes, TC genotype was dominant genotype (0.51), C was the dominant allele (0.55). χ² test indicated that g. 13504098 C>T, g. 11204707 A>C and g. 18795097 C>T of Ujumqin sheep were in Hardy-Weinberg equilibrium (P>0.05). Population genetic analysis showed that g. 13504098 C>T, g. 11204707 A>C and g. 18795097 C>T were at moderate polymorphisms in Ujumqin sheep population (0.25<PIC<0.50). Association analysis revealed that g. 13504098 C>T was extremely significantly associated with the body weight, body height, chest girth, shin circumference of Ujumqin sheep at 4 and 6 months of age, and the chest width at 6 months of age (P<0.01);g. 11204707 A>C was extremely significantly associated with the body height and body length of Ujumqin sheep at 4 and 6 months of age, the body weight at 4 months of age, and the chest girth at 6 months of age (P<0.01), and it was significantly associated with the body weight and shin circumference at 6 months of age (P<0.05);g. 18795097 C>T was extremely significantly associated with the body height at 4 months of age and the chest girth at 6 months of age (P<0.01), and significantly associated with the body weight and chest girth at 4 months of age, and the chest width at 6 months of age (P<0.05). Therefore, it could be concluded that g. 13504098 C>T of DGAT1 gene, g. 11204707 A>C of NR6A1 gene and g. 18795097 C>T of MAN1A1 gene had significantly correlation with the growth traits of Ujumuqin sheep, indicating that 3 SNPs could be used as candidate molecular markers for genetic breeding in Ujumuqin sheep.

Key words: sheep; DGAT1 gene; NR6A1 gene; MAN1A1 gene; growth traits

CLC Number:

S813.3

ZHANG Jinhua, SHANG Mingyu, HU Wenping, ZHANG Li. Association Analysis Between Polymorphisms of DGAT1,NR6A1 and MAN1A1 Genes and Growth Traits in Ujumqin Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3312-3322.

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Association Analysis Between Polymorphisms of DGAT1,NR6A1 and MAN1A1 Genes and Growth Traits in Ujumqin Sheep

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