羊口疮病毒贵州株F1L基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2015.01.008

Abstract

Abstract: In order to study and analyze the F1L gene of Orf virus in Guizhou province (ORFV-GZ), we studied the scab materials of lambs with clinical sore mouth symptom in Guizhou province.The F1L gene was amplified, cloned and sequenced using bioinformatics softwares and methods, the secondary structure, B-cell preponderant epitope, conserved domains analysis, transmembrane domain and signal peptide of F1L gene were predicted.The results indicated the length of F1L gene was 1 029 bp, encoding 342 amino acids.The F1L gene of ORFV-GZ strain shared a nucleotide identities of 98.4%, 97.9%, 97.8% and 96.8%, and an amino acid identities of 98.3%, 97.6%, 97.3% and 95.3% with those of strains OV-SA00, NZ2, OV-IA82 and D1701, respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between ORFV-GZ strain and FJ-GT.Prediction of the secondary structure of F1L indicated that the alpha helix and random coil took a higher percentage.The F1L protein was supposed contain 7 potential antigen epitopes, and two transmembrane domains, no signal peptide found.These results provided a theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of ORFV.

Key words: Orf virus (ORFV); F1L gene; clone; bioinformatics analysis

CLC Number:

S858.26

LIU Ai, YANG Yu, XIAN Si-mei, LIU Zong-sheng, WU Jian, LUO Bo, CHEN Yong-cui. Cloning and Bioinformatic Analysis of F1L Gene of Orf Virus from Guizhou Province[J]. , 2015, 42(1): 53-60.

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Cloning and Bioinformatic Analysis of F1L Gene of Orf Virus from Guizhou Province

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