鹅细小病毒VP3基因在昆虫杆状病毒表达系统中的表达

Abstract

Abstract: The fusion VP3 gene sequence of goose parvovirus (GPV) was amplified by PCR using a pair of specific primers from GPV FY89 strain.The PCR product was cloned into pFastBac/HBM-TOPO vector and transformed into DH10Bac cells.Recombinant Bacmid-VP3 was transfected into Sf9 insect cells，and a fusion protein about 61 ku was expressed and identified by SDS-PAGE analysis.The antigenicity of the recombinant VP3 protein was confirmed by Western blotting.

Key words: goose parvovirus (GPV); VP3 gene; baculovirus expression system

CLC Number:

Q785

WANG Zhi-qiang，LI Hong-bin，YANG Xu-dong，HUANG Yu-xiang，ZOU Yue，ZHANG Jun，LIU Li-wei. Expression of Goose Parvovirus VP3 Gene in Baculovirus Expression System[J]. .

References

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Expression of Goose Parvovirus VP3 Gene in Baculovirus Expression System

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