抗氧氟沙星单克隆抗体的制备及ELISA快速试剂盒的初步研制

Abstract

Abstract: Ofloxacin was coupled with BSA as an immunogen and with OVA as a coating antigen using the coupling agent EDC. Monoclonal antibodies against ofloxacin and its enzyme linked immunosorbent assay kit were prepared and established,respectively. The limit of detection was 1 μg/kg in fish, and the recovery range was 62.8% to 97.7%. The intra and inter assay RSD were both less than 10%.

Key words: ofloxacin; monoclonal antibodies; enzyme linked immunosorbent assay kit; rapid detection

DONG Li-xue，FENG Cai-wei，FENG Jing，ZHENG Bai-qin，JIA Fang-fang，MENG Jun-li，HAO Li-wu，ZHANG Shu-hong. Preparation of Monoclonal Antibodies against Ofloxacin and Preliminary Development of its Enzyme Linked Immunosorbent Assay Kit for Rapid Detection[J]. China Animal Husbandry & Veterinary Medicine.

References

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Preparation of Monoclonal Antibodies against Ofloxacin and Preliminary Development of its Enzyme Linked Immunosorbent Assay Kit for Rapid Detection

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