弓形虫DOC2基因C-端的克隆及序列分析

Abstract

Abstract: In order to evaluate whether Toxoplasma gondii DOC2 gene could be used as a vaccine candidate antigen, the C-terminal of DOC2 gene was amplified by RT-PCR and then sent for sequencing. After translated the gene sequence into amino acid sequence, the basically biological characters, advanced structures and linear B cell epitopes of the C-terminal of DOC2 gene were predicted using multiple bioinformatics analysis. The results showed that the length of C-terminal of DOC2 gene was 1887 bp. The C-terminal of DOC2 protein was predicted contain 11 hydrophilic regions, 7 transmembrane domains,19 alpha regions, 1 beta turn, 17 random coils and 12 B cell epitopes, which suggested this protein was a probable vaccine candidate against Toxoplasma gondii infection.

Key words: Toxoplasma gondii; DOC2 gene; clone; bioinformatics analysis

GAO Qi, ZHANG Nian-zhang, HU Ling-ying, ZHOU Dong-hui,ZHU Xing-quan,WENG Ya-biao. Cloning and Sequence Analysis of C-terminal of Toxoplasma gondii DOC2 Gene[J]. .

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Cloning and Sequence Analysis of C-terminal of Toxoplasma gondii DOC2 Gene

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