泰山枯草芽孢杆菌的分离鉴定及其对黄曲霉毒素B1的降解研究

Abstract

Abstract: The aim of this experiment was to select the Bacillus subtilis which was useful for the degradation of aflatoxin B₁ from the soil of Mountain Tai.Through the 16S rRNA gene sequencing，we identified one Bacillus subtilis which had high degradation effect of aflatoxin B₁，named as Mountain Tai Bacillus subtilis，the determination of degradation efficiency and active components of aflatoxin B₁ and the effect of degradation aflatoxin in feed were researched in this experiment used Mountain Tai Bacillus subtilis.The results showed that the degradation rate of aflatoxin B₁ of Mountain Tai Bacillus subtilis was 90.28%，the degrading efficiencies of aflatoxin B₁ by exocellular coarse extraction liquid was highest，the activity of aflatoxins degradation mainly happened in the culture supernatant of Mountain Tai Bacillus subtilis.Characteristic research of active component including heating and degeneration of proteinase K，the detoxification decreased significantly，which suggested that the effect of supernatant from Mountain Tai Bacillus subtilis on aflatoxin B₁ might be due to the enzymatic reactions.Further application tests proved that Mountain Tai Bacillus subtilis could degrade aflatoxin B₁ in mouldy feed and it could be applied in the feed or food industry.

Key words: Bacillus subtilis; isolation and identification; aflatoxin B₁（AFB₁）; culture supernatant; degradation enzyme

SUN Ling-yu，LI Chao，HAO Hai-yu，CHAI Tong-jie. Study on Isolation and Identification of Mountain Tai Bacillus subtilis and its Degradation of Aflatoxin B₁[J]. .

References

1 东秀珠，蔡妙英.常见细菌系统鉴定手册［M］.北京：科学出版社，2001.
2 张海涛，王加启，卜登攀，等.日粮中添加纳豆枯草芽孢杆菌对犊牛消化道发育的影响［J］.中国畜牧兽医，2010，37（1）：5～9.
3 章挺，胡梁斌，王飞，等.拮抗菌B-FS06的鉴定及其发酵产物对黄曲霉的抑制作用［J］.中国生物防治，2007，23（2）：160～165.
4 雷元培，赵丽红，马秋刚，等.降解黄曲霉毒素枯草芽孢杆菌的解毒性、抗菌性及抗逆性研究［J］.饲料工业，2012，32（24）：23～27.
5 黄雪泉.添加枯草芽孢杆菌制剂对仔猪生产性能的影响［J］.中国畜牧兽医，2010，37（7）：212～214.
6 Alberts J F，Engelbrecht Y，Steyn P S，et al.Biological degradation of aflatoxin B₁ by Rhodococcus erythropolis cultures［J］.International Journal of Food Microbiology，2006，109（1）：121～126.
7 Ciegler A，Lillehoj E B，Peterson R E，et al.Microbial detoxification of aflatoxin［J］.Applied Microbiology，1966，14（6）：934～939.
8 Cole R J，Dorner J W.Extraction of aflatoxins from naturally contaminated peanuts with different solvents and solvent/peanut ratios［J］.Journal of AOAC International，1993，77（6）：1509～1511.
9 DeLong E F.Archaea in coastal marine environments［J］.Proceedings of the National Academy of Sciences，1992，89（12）：5685～5689.
10 Desheng Q，Fan L，Yanhu Y，et al.Adsorption of aflatoxin B₁ on montmorillonite［J］.Poultry Science，2005，84（6）：959～961.
11 El-Nezami H，Kankaanpaa P，Salminen S，et al.Ability of dairy strains of lactic acid bacteria to bind a common food carcinogen，aflatoxin B₁［J］.Food and Chemical Toxicology，1998，36（4）：321～326.
12 Gowda N K S，Suganthi R U，Malathi V，et al.Efficacy of heat treatment and sun drying of aflatoxin-contaminated feed for reducing the harmful biological effects in sheep［J］.Animal Feed Science and Technology，2007，133（1）：167～175.
13 Hormisch D，Hormisch D，Brost I，et al.Mycobacterium fluoranthenivorans sp. nov.，a fluoranthene and aflatoxin B₁ degrading bacterium from contaminated soil of a former coal gas plant［J］.Systematic and Applied Microbiology，2004，27（6）：653～660.
14 Kohli E，Raj H G，Kumari R，et al.Comparison of the prevention of aflatoxin B₁-induced genotoxicity by quercetin and quercetin pentaacetate［J］.Bioorganic & Medicinal Chemistry Letters，2002，12（18）：2579～2582.
15 Mukendi N，Rollmann B，de Meester C.Detoxification of aflatoxin B₁ by different chemical methods and evaluation of the effectiveness of the treatments applied［J］.Journal de Pharmacie de Belgique，1990，46（3）：182～188.
16 Peltonen K D，El-Nezami H S，Salminen S J，et al.Binding of aflatoxin B₁ by probiotic bacteria［J］.Journal of the Science of Food and Agriculture，2000，80（13）：1942～1945.
17 Teniola O D，Addo P A，Brost I M，et al.Degradation of aflatoxin B₁ by cell-free extracts of Rhodococcus erythropolis and Mycobacterium fluoranthenivorans sp. nov. DSM44556T［J］.International Journal of Food Microbiology，2005，105（2）：111～117.
18 Yousef A E，Marth E H.Use of ultraviolet energy to degrade aflatoxin M₁ in raw or heated milk with and without added peroxide［J］.Journal of Dairy Science，1986，69（9）：2243～2250.
19 Zhao L H，Guan S，Gao X，et al.Preparation，purification and characteristics of an aflatoxin degradation enzyme from Myxococcus fulvus ANSM068 ［J］.Journal of Applied Microbiology，2011，110（1）：147～155.

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[3]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[4]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[5]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[6]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[7]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[8]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[9]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.
[10]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.
[11]	XI Jing, YI Jihai, WANG Yueli, XU Zhenyu, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu. Isolation, Identification and Genotype Analysis of Group A Bovine Rotavirus G10 Strain XJ-2022 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3530-3538.
[12]	LIU Xiaolan, LIU Changjin, YU Wenyang, LI Xiaoxiang, BIAN Yanchao, HUANG Xiaohua, LUO Feng, DENG Shunzhou. Isolation and Identification of Porcine Rotavirus Jiangxi Strain AY01 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3151-3162.
[13]	XU Minsheng, KE Haiyi, SHI Keda, YANG Dongxia, ZHAI Shaolun, ZANG Yingan, LI Chunling. Isolation and Identification of Actinobacillus pleuropneumoniae from Guangdong Province and Detection and Analysis of Drug Resistance Phenotype and Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3212-3225.
[14]	YANG Zemin, LI Shuang, JIN Zhengyu, LIAO Yixiao, YANG Ying, WEN Ming. Isolation,Identification and Biological Characteristics of a Strain of Lactobacillus plantarum from Carp [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2805-2811.
[15]	YIN Yajie, LIANG Ruiying, CUI Huan, MENG Lijia, NI Weiling, QIAO Sina, GUO Kangkang, ZHANG Bo, LI Songli, HOU Shaohua, DONG Shishan. Isolation,Identification of Duck Tembusu Virus,and Genetic and Evolutionary Analysis of Its Envelope Protein [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1497-1506.

Study on Isolation and Identification of Mountain Tai Bacillus subtilis and its Degradation of Aflatoxin B₁

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Study on Isolation and Identification of Mountain Tai Bacillus subtilis and its Degradation of Aflatoxin B1

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Study on Isolation and Identification of Mountain Tai Bacillus subtilis and its Degradation of Aflatoxin B₁