山羊腹泻粪便中奇异变形杆菌的分离鉴定及系统发育分析

Abstract

Abstract: Proteus mirabilis were identified from goat manure samples with diarrhea from a goat farm in Chengdu of Sichuan province (14/20). 5 isolates (5×10^⁸ CFU each) were randomly selected from 17 Proteus mirabilis and infected into mice by intraperitoneal injection. The results showed that all of the injected mice were dead within 12 h. 16S rRNA genes of Proteus mirabilis isolates were sequenced and analyzed. The genes shared 99% to 100% homology with the sequence of Proteus mirabilis available in GenBank， and shared a homology of 99.8% to 100% within isolates in this study. Phylogenetic analysis showed that isolates clustered into two branches and presented some diversity, but no significant correlation of evolution between different areas and hosts. In conclusion, Proteus mirabilis was closely involved in goat diarrhea. Its harm to the sheep industry and public health significance should be seriously concerned.

Key words: goats; diarrhea; Proteus mirabilis; isolation and identification; 16S rRNA; phylogenetic analysis

CHEN Xi, WANG Yuan-wei, WANG Yong, TANG Cheng. Isolation， Identification and Phylogenetic Analysis of Proteus mirabilis from Goats with Diarrhea[J]. .

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Isolation， Identification and Phylogenetic Analysis of Proteus mirabilis from Goats with Diarrhea

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