Ⅰa型牛源无乳链球菌LZQ07006分离株BibA基因片段的分子特征分析

Abstract

Abstract: This study was aimed at analyzing the molecular characteristics of BibA gene and its expression protein, providing theoretical basis for the further study on the expression and immunogenicity of BibA protein. The BibA gene of the isolate LZQ07006 of serotype Ⅰa Streptococcus agalactiae from bovine was amplified using the PCR method, cloned into pMD20-T vector and then sequenced. The molecular characteristics of BibA gene and its expression protein were analyzed using a variety of biological software. The results showed that BibA gene of LZQ07006 was 1185 bp and did not appear the gene deletion, and encoded 395 amino acids. The nucleotide sequence homology of BibA gene derived from LZQ07006 was 45.0% to 99.8% and the amino acid homology was 12.3% to 99.5% among the corresponding sequences from different serotypes Streptococcus agalactiae published in GenBank. The derived amino acid showed that the BibA protein was a stable outer membrane protein, which was secretion protein and hydrophilic highly. The polypeptide contained a signal peptide consisting of 32 amino acids, with cleavage site between 32th and 33th amino acids. In conclusion, it was necessary to study the BibA protein in bovine Streptococcus agalactiae for application in diagnosis or prevention of dairy cow mastitis.

Key words: font-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">serotype font-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">Ⅰa; Streptococcus agalactiae; BibA gene; clone; sequence analysis

CHANG Rui-xiang, WANG Xu-rong, WANG Lei, ZHANG Jing-yan, QIN Zhe, KONG Xiao-jun, WANG Xiao-wu, LI Jian-xi, YANG Zhi-qiang, WANG Xue-zhi. Molecular Characterization Analysis of BibA Gene in the Isolate LZQ07006 of Serotype Ⅰa Streptococcus agalactiae from Bovine[J]. .

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Molecular Characterization Analysis of BibA Gene in the Isolate LZQ07006 of Serotype Ⅰa Streptococcus agalactiae from Bovine

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