胆汁酸膜受体TGR5真核表达载体的构建及在细胞中的表达

Abstract

Abstract: The study was aimed to construct a membrane-bound bile acid receptor TGR5 eukaryotic expression vector, which transfected into 293T cell, and expressed in 293T cell. Total RNA was extracted from placenta tissue, and RT-PCR was performed to obtain the TGR5 cDNA, which was inserted into the eukaryotic expression vector pCMV-EGFP. The novel constructed plasmid was confirmed by restriction enzyme digestion and DNA sequencing, then 293T cell was transfected with pCMV-EGFP-TGR5, Real-time PCR and Western blotting were amplified to determine the expression of TGR5. The result showed that the eukaryotic expression vector of pCMV-EGFP-TGR5 was constructed successfully. Green fluorescent was examined under fluorescent microscope, and the expression of TGR5 was increased significantly in 293T cells. The eukaryotic expression vector pCMV-EGFP-TGR5 was constructed successfully and TGR5 was expressed in mRNA and protein levels in 293T cell after transfection.

Key words: font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">TGRfont-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">5 gene; clone; construction of eukaryotic expression vector

SHI Lin-kai, LIU Qian, ZHANG Zi-ying, LIU Chang， WANG Xu-dong, DING Yue-xia, LI Pei-feng. Construction of Bile Acid Receptor TGR5 Eukaryotic Expression Vector and its Expression in Cell[J]. .

References

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Construction of Bile Acid Receptor TGR5 Eukaryotic Expression Vector and its Expression in Cell

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