牛粪中纤维素分解菌的分离鉴定及其产酶条件优化研究

Abstract

Abstract: :A strain of highly cellulolytic decomposing bacteria was obtained by the congo red flat plate straining and shake flasking method screened from the ox dung which named N12. It had been identified as Bacillus which belonged to Bacillus pumilus sp. by morphologic characteristics observation，physiology and biochemical experiment and also 16S rRNA genetic analyze.The conditions of producing cellulase on strain N12 were also been studied in this test.When the contents of carbon and nitrogen sources were 0.5% maizena and 1% yeast powder，and also the nitial pH was 3.0，the fermentation temperature was 42 ℃,at the same time the fermentation time was 54 h，it had the highest enzyme activity.

Key words: ox dung; cellulose decomposing bacteria; identification; conditions optimize

GAO Yun-hang，ZHANG Xi-hong，WANG Wei，WANG Yan-qiu，MA Hong-xia，LOU Yu-jie. Study on Isolation，Identification and Enzyme-producing Conditions Optimized of Cellulolytic Bacteria in Ox Dung[J]. .

References

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Study on Isolation，Identification and Enzyme-producing Conditions Optimized of Cellulolytic Bacteria in Ox Dung

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