新型重配A（H7N9）流感病毒NA基因序列分析

Abstract

Abstract: The assay was aimed to analyze the molecular epidemiological character of influenza A (H7N9) virus in 2013. The complete genome sequences of NA of influenza virus strains from different discrete host sources downloaded from the GenBank were analyzed on genetic evolution by DNAStar software in this research. The results showed that, the neck amino acids of NA in influenza A (H7N9) virus in 2013 was deleted; HB site of NA took adaptive changes in certain degrees; the active site of proteases of HA with seven glycosylation sites was unchanged; the top ten high nucleotide homology strains with HA of A/Hangzhou/1/2013(H7N9) had isolated from poultry in five Asian countries, the nucleotide homology was more than 96%. It was not clear whether the NA gene of novel reassortment influenza A (H7N9) virus strains was avian-to-human transmission and needed further study.

Key words: influenza A virus; neuraminidase; genetic evolution analysis

LIU Yong-hong, ZHAO Li, DAI Yan-yan. Sequence Analysis of NA Gene of the Novel Reassortment Influenza A (H7N9) Virus[J]. China Animal Husbandry & Veterinary Medicine.

References

1 王守春，尹燕博，王晓红，等．63株H9N2亚型禽流感病毒NA基因序列分析[J]．畜牧兽医学报，2011，42(2)：297～305.
2 王泽霖，刘岩，李建丽，等．H9N2亚型禽流感病毒抗原性变异的研究[J]．中国兽医学报，2007，27(3)：351～354.
3 刘金华，史为民，吴清民，等．鸡源H9N2亚型流行性感冒病毒神经氨酸酶基因序列分析[J]．病毒学报，2004，20(3)：237～241.
4 孙晓东，熊永忠，陈化兰，等.流感病毒抗原表位的研究进展[J].中国畜牧兽医，2011，38(6)：193～196.
5 朱闻斐，高荣保，王大燕，等．H7亚型禽流感病毒概述[J]．病毒学报，2013，29(3)：245～249.
6 杨春，余佳，徐凤琴，等．甲型流感病毒抗原变异的机理[M]．国外医学病毒学分册，1999，6(3)：88～90.
7 苏敬良，高福，索勋主译．禽病学[M]．第11版. 北京：中国农业出版社，2005.
8 武斌，王承明，董国英，等．甲型H1N1流感病毒北美毒株的分子特征[J]．科学通报，2009，54(121)：1661～1672.
9 侯云德．分子病毒学[M]．北京：学苑出版社，1990.
10 殷震，刘景华．动物病毒学[M]．第2版. 北京：科学技术出版社，1997.
11 郭元吉，程小雯．流行性感冒及其实验技术[M]．北京：中国三峡出版社，1997.
12 郭霄峰，廖明，辛朝安．禽流感病毒KMI/99(H9N2)HA和NA基因的序列分析[J]．畜牧兽医学报，2002a，33(5)：486～491.
13 郭霄峰，廖明，辛朝安．H9N2亚型禽流感病毒纤突蛋白基因的分析[J]．中国人兽共患病杂志，2002b，18(4)：17～19.
14 舒跃龙主编．郭元吉论文集[M]．北京：中国科学技术出版社，2006.
15 Both G W, Sleigh M J. Conservation and variation in the haemagglutinins of Hong Kong subtype influenza viruses during antigenic drift[J]. J Virol 1981, 39(3): 663～672.
16 Fouchier R A, Munster V, Wallensten A, et al. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls[J]. Journal of Virology, 2005, 79(5):2814～2822.
17 Goto H, Kawaoka Y. A novel mechanism for the acquisition of virulence by a human influenza A virus[J]. Proc Natl Acad Sci USA, 1998, 95: 10224～10228.
18 Hilleman M. Realities and enigmas of human viral influenza: Pathogenesis, epidemiology and control[J]. Vaccine, 2002, 20(25～26): 3068～3087.
19 http://health. sohu. com/s2013/qlg/[OL].
20 http://society. people. com. cn/GB/10472345. html[OL].
21 http://www. who. int/csr/don/2010_05_07/en/index. html[OL].
22 Hurt A C, Holien J K, Parker M, et al. Zanamivir-resistant influenza viruses with a novel neuraminidase mutation[J]. J Virol, 2009, 83(20): 10366～10373.
23 Iqbal M, Yaqub T, Reddy K, et al. Novel genotypes of H9N2 influenza A viruses isolated from poultry in Pakistan containing NS genes similar to highly pathogenic H7N3 and H5N1 viruses[J]. PLoS One, 2009, 4(6): e5788.
24 Katz J M, Lu X H, Tumpey T M, et al. Molecular correlates of influenza A H5N1 virus pathogenesis in mice[J]. Virology, 2000, 74(22): 10807～10810.
25 Luo G, Jeffrey C, Palease P. Alteration of the stalk of the influenza virus neuraminidase: Deletions and insertions[J]. Virus Ras, 1993, 29(2): 141～153.
26 Matrosovich M N, Krauss S, Webster R G. H9N2 influenza A viruses from poultry in Asia have human virus-like receptor specificity[J]. Virology, 2001, 281(2): 156～162.
27 Matrosovich M, Zhou N, Kawaoka Y, et al. The surface glycoproteins of H5 influenza viruses isolated from humans, chickens, and wild aquatic birds have distinguishable properties[J]. J Virol, 1999, 73(2): 1146～1155.
28 McKimm-Breschkin J L. Resistance of influenza viruses to neuraminidase inhibitors——A review[J]. Antiviral Res, 2000, 47(1): 1～17.
29 Perk S, Banet-Noach C, Shihmanter E, et al. Genetic characterization of the H9N2 influenza viruses circulated in the poultry population in Israel[J]. Comp Immunol Microbiol Infect Dis, 2006, 29(4): 207～223.
30 Potter C W. A history of influenza[J]. J Appl Microbiol, 2001, 91(4): 572～579.

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Sequence Analysis of NA Gene of the Novel Reassortment Influenza A (H7N9) Virus

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