京海黄鸡钠氢离子交换泵1基因实时荧光定量PCR标准曲线的建立

Abstract

Abstract: The purpose of this study was to elucidate the Na⁺/H⁺ exchanger 1 （NHE1） gene expression and its normal physiological functions. The primers were designed and synthesized based on the nucleotide sequences of NHE1 gene available in GenBank. The 204 bp fragments were amplified by RT-PCR from mRNA of Jinghai Yellow chickens, and then the fragments were cloned and sequenced. The recombinant plasmids were diluted by 10-fold serial and used as the Real-time PCR standard templates. The constructed standard curve had a relative coefficient R² = 0.9903 and the regressing equation:y =-3.4643x+35.9000 suggested it was successful, and they provided powerful tools for quantification of mRNA of NHE1 gene in Jinghai Yellow chickens.

Key words: Na⁺/H⁺ exchanger 1; Jinghai Yellow chicken; recombinant plasmid; expression; Real-time PCR

CHEN Xue-sen, WANG Jin-yu, ZHANG Gen-xi, ZHANG Tao, TANG Ying, SHI Hui-qiang. Establishment of Standard Curves for Detection of Na⁺/H⁺ Exchanger 1 Gene in Jinghai Yellow Chicken Using Real-time PCR[J]. .

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Establishment of Standard Curves for Detection of Na⁺/H⁺ Exchanger 1 Gene in Jinghai Yellow Chicken Using Real-time PCR

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Establishment of Standard Curves for Detection of Na+/H+ Exchanger 1 Gene in Jinghai Yellow Chicken Using Real-time PCR

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Establishment of Standard Curves for Detection of Na⁺/H⁺ Exchanger 1 Gene in Jinghai Yellow Chicken Using Real-time PCR